...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Iranian journal of public health. >Use of Single-enzyme PCR-restriction Digestion Barcode Targeting the Internal Transcribed Spacers (ITS rDNA) to Identify Dermatophyte Species
【24h】

Use of Single-enzyme PCR-restriction Digestion Barcode Targeting the Internal Transcribed Spacers (ITS rDNA) to Identify Dermatophyte Species

机译：使用针对内部转录间隔子（ITS rDNA）的单酶PCR限制性消化条形码来鉴定皮肤癣菌物种

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Dermatophytes are the most common causative agents of superficial mycoses. Species identification of these fungi is important from therapeutic and epidemiological point of wive. Traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstances. Recently a broad varieties of rapid and accurate DNA-based techniques were successfuly utilized for species delineation of dermatophytes.Methods: The ITS1-5.8S-ITS2 region of rDNA from various reference strains of dermatophyte species were amplified using the universal fungal primers ITS1 and ITS4.The PCR products were digested by a single restriction enzyme, Mval. The enzyme was evaluated in both in silico and practical PCR-RFLP assay to find the exact differentiating restriction profiles for each species. To validate the standardized PCR-RFLP system, all tested strains were subjected to sequencing and sequence analysis.Results: The obtained RFLP patterns were specific for many species including T. interdigitale, T. rubrum, T. violaceum, M. persicolor, M. audouinii, M. nanum (A. obtusum) and E. floccosum but were similar for some closely related species such as M. canis I M. ferrugineum. Sequencing of the ITS1-5.8S-ITS2 fragment from all type strains affirmed the RFLP findings.Conclusion: It was practically revealed that the ITS-PCR followed by Mval-RFLP is a useful and reliable schema for identification and differentiation of several pathogenic species and can be used for rapid screening of even closely related species of dermatophytes in clinical and epidemiological settings.
机译：背景：皮肤癣菌是浅表真菌病最常见的病原体。从妻子的治疗和流行病学角度出发，对这些真菌进行物种鉴定非常重要。依靠菌落的宏观和微观特征在物种水平上鉴定皮肤真菌的传统方法通常是耗时的，并且在许多情况下都不可靠。最近，成功地将各种各样基于快速，准确的基于DNA的技术用于皮癣菌的物种鉴定。方法：使用通用真菌引物ITS1和ITS4扩增了来自各种皮癣菌参考菌株的rDNA的ITS1-5.8S-ITS2区。 PCR产物用单个限制酶Mval消化。在计算机模拟和实际PCR-RFLP分析中均评估了该酶，以找到每种物种的确切差异限制性内切酶谱。为了验证标准化的PCR-RFLP系统，对所有测试菌株进行测序和序列分析。结果：获得的RFLP图谱对许多物种具有特异性，包括指叉毛线虫，红毛线虫，紫毛线虫，百日草，杂色线虫。 audouinii，M。nanum（A. obtusum）和E. floccosum，但在一些密切相关的物种（如犬莫里斯犬I. Ferrugineum）上相似。对所有类型菌株的ITS1-5.8S-ITS2片段进行测序证实了RFLP的发现。结论：实际表明，ITS-PCR和Mval-RFLP结合在一起是鉴定和区分几种致病菌的有用且可靠的方案。可用于在临床和流行病学环境中快速筛选甚至紧密相关的皮肤真菌物种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号