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首页> 外文期刊>Italian journal of animal science >Effect of oocyte selection, estradiol and antioxidant treatment on in vitro maturation of oocytes collected from prepubertal Boer goats
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Effect of oocyte selection, estradiol and antioxidant treatment on in vitro maturation of oocytes collected from prepubertal Boer goats

机译：卵母细胞选择，雌二醇和抗氧化剂处理对青春期前波尔山羊卵母细胞体外成熟的影响

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摘要

Development of improved procedures for in vitro maturation of oocytes collected from prepubertal goats has applications for in vitro embryo production and accompanying strategies for genetic improvement. The objective of described studies was to determine the effects of oocyte grade, in vitro maturation time, antioxidant supplementation and concentrations of estradiol in the maturation medium on in vitro maturation of oocytes harvested from 16 mm follicles present on the ovaries (obtained from an abattoir) of 1-6-month old prepubertal Boer goats. Rates of progression to metaphase II were greater for Grade 1 oocytes (>3 compact layers of cumulus cells and evenly granulated cytoplasm) than Grade 2 oocytes (<3 layers cumulus cells and evenly granulated cytoplasm) and were lowest for Grade 3 oocytes (devoid of cumulus cells with abnormal cytoplasm). No significant effects of maturation for 24 vs 27 vs 30 h on progression of oocytes to metaphase II were observed. The addition of 5 mu M beta-mercaptoethanol to maturation medium increased the proportion of oocytes progressing to metaphase II and glutathione content in such oocytes, whereas supplementation with 10 mM hypotaurine was without effect. A significant inhibitory effect of in vitro maturation in the presence of high concentrations of estradiol (10 and 100 mu g/mL) on progression to metaphase II was observed, and no effect was observed in response to 1 mu g/mL estradiol treatment as compared with control. Results suggest that oocyte selection and beta-mercaptoethanol supplementation can positively influence progression to metaphase II of oocytes harvested from ovaries of prepubertal goats, whereas high concentrations of estradiol are inhibitory to in vitro maturation.
机译：从青春期前山羊中收集的卵母细胞体外成熟的改良方法的开发，已经在体外胚胎生产和遗传改良策略中得到了应用。所描述研究的目的是确定卵母细胞等级，体外成熟时间，抗氧化剂的补充以及成熟培养基中雌二醇的浓度对从卵巢上存在的16毫米卵泡（从屠宰场获得）收获的卵母细胞体外成熟的影响。 1-6个月大的青春期前布尔山羊。 1级卵母细胞（> 3个紧密层的卵丘细胞和均匀的颗粒状细胞质）进展到中期II的速率高于2级卵母细胞（<3层卵丘细胞和均匀粒状的细胞质），而3级卵母细胞（没有卵母细胞）最低胞质异常的卵丘细胞）。没有观察到成熟24小时，27小时和30小时对卵母细胞进展到中期II的显着影响。向成熟培养基中添加5μMβ-巯基乙醇可增加卵母细胞进入卵母细胞进入中期II的比例和谷胱甘肽含量，而补充10 mM次牛磺酸则无效果。观察到在高浓度雌二醇（10和100μg / mL）存在下体外成熟对进展至中期II的显着抑制作用，与​​之相比，对1μg / mL雌二醇处理未观察到任何抑制作用与控制。结果表明，卵母细胞的选择和β-巯基乙醇的补充可以积极地影响从青春期前山羊卵巢中收获的卵母细胞到中期II的进程，而高浓度的雌二醇则对体外成熟具有抑制作用。

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