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首页> 外文期刊>Israel Journal of Plant Sciences >Watermelon chlorotic stunt and Squash leaf curl begomoviruses—New threats to cucurbit crops in the Middle East
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Watermelon chlorotic stunt and Squash leaf curl begomoviruses—New threats to cucurbit crops in the Middle East

机译：西瓜绿化特技和南瓜卷叶型begomoviruses-对中东葫芦类作物的新威胁

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摘要

During the year 2002, two new diseases with unknown etiologies were detected in cucurbit crops in Israel. One disease was detected in squash fields throughout the country, while the second appeared in a single watermelon plot in the south, just outside the city of Elat. The infected watermelon plot was eradicated, but nonetheless the new disease spread throughout the country and today it is present in all the watermelon production areas. Both diseases were associated with elevated whitefly populations. Indeed, it was found that both are transmitted only by whiteflies, and are incited by two begomoviruses. Both viruses were cloned and sequenced, and were identified as Watermelon chlorotic stunt virus (WmCSV) and Squash leaf curl virus (SLCV). The host range of the Israeli WmCSV isolate (WmCSV-IL) was determined and resembles the host range of the three other known WmCSV isolates (from Yemen, Sudan, and Iran), but with a few differences. Although no genetic resistance to WmCSV was observed in cultivated watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai], resistance to the virus was found in a wild relative, colocynth (Citrullus colocynthis (L) Schrader). Nucleotide sequence comparisons revealed that WmCSV-IL is highly homologous to other WmCSVisolates, with the highest homology (nearly identical) to the isolate from Sudan. SLCV-IL host range was determined as well, and was also found to be similar to other SLCV isolates. However, following genome sequencing, it was found that due to two separate point mutations, two viral open reading frames (ORF) were altered. The AC2 ORF was extended by 129 nucleotides, while the BV1 ORF was reduced by 99 nucleotides.
机译：在2002年期间，在以色列的葫芦类作物中发现了两种病因不明的新疾病。在全国各地的南瓜田中发现了一种疾病，而第二种疾病则出现在南部埃拉特市外的一个西瓜田中。虽然已消灭了被感染的西瓜地，但新的疾病仍在全国蔓延，如今已在所有西瓜产区出现。两种疾病均与粉虱种群增加有关。确实，发现两者都仅由粉虱传播，并且由两种begomoviruss激发。两种病毒均被克隆和测序，并被鉴定为西瓜绿叶病特技病毒（WmCSV）和南瓜弯叶病毒（SLCV）。确定了以色列WmCSV分离株（WmCSV-IL）的宿主范围，与其他三个已知的WmCSV分离株（也门，苏丹和伊朗）的宿主范围相似，但有一些区别。尽管在栽培的西瓜[Citrullus lanatus（Thunb。）Matsum]中未观察到对WmCSV的遗传抗性。 ＆Nakai]，在野生亲缘种（Citrullus colocynthis（L）Schrader）中发现了对该病毒的抗性。核苷酸序列比较显示，WmCSV-IL与其他WmCSV分离株高度同源，与苏丹分离株的同源性最高（几乎相同）。还确定了SLCV-IL宿主范围，也发现其与其他SLCV分离株相似。然而，在基因组测序之后，发现由于两个单独的点突变，两个病毒开放阅读框（ORF）被改变。 AC2 ORF扩展了129个核苷酸，而BV1 ORF降低了99个核苷酸。

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