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首页> 外文期刊>Israel Journal of Plant Sciences >Expression profiling and characterization of cold, freezing-related genes from Brassica rapa cultivars
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Expression profiling and characterization of cold, freezing-related genes from Brassica rapa cultivars

机译：甘蓝型油菜冷，冰冻相关基因的表达谱分析和鉴定

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Brassica is a very important vegetable group worldwide. Cold and freezing stress are the major environmental factors that limit the productivity of Brassica. In this study, we retrieved 903 stress-chilled unigenes and the unique transcripts were classified functionally using gene ontology (GO) hierarchy, Kyoto Encyclopedia of Genes and Genomes (KEGG). KEGG orthology and structural domain data were obtained from the biological database to the unigene data set. Unigene data sets provide a wide outlook of functional characterization of Brassica rapa. In silico analysis revealed 94.2% of unigenes to be well annotated toward Reeds one-dimensional concept. On the basis of similarity searches and GO annotation of biological process (BP), 139, 113, and 57 sequences showed a response to stress (SR), abiotic stimulus (AS), and biotic stimulus (BS), respectively. To validate this observation, seven unigenes were randomly selected from AS that are known to be associated with cold stress from previous studies in other species and these genes were examined by real-time polymerase chain reaction to understand the change in expression pattern under cold and freezing stress. In silico study of occurrence in the AS subcategory revealed the presence of 138 single sequence repeats, some of which are being explored to assess genetic diversity among B. rapa. Thus, the unigene data set provides valid resources for discovering the potential genes related to cold and freezing resistance in B. rapa cultivars, and can be useful resources for genetic engineering of Brassica sp.
机译：芸苔属是世界范围内非常重要的蔬菜群。低温和冰冻压力是限制芸苔属植物生产力的主要环境因素。在这项研究中，我们检索了903个压力冷的单基因，并使用基因本体论（GO）层次，《京都基因与基因组百科全书》（KEGG）对独特的转录本进行了功能分类。从生物学数据库到unigene数据集获取KEGG的正字学和结构域数据。 Unigene数据集为甘蓝型油菜的功能表征提供了广阔的前景。通过计算机分析，发现94.2％的单基因对Reeds一维概念有很好的注释。根据相似性搜索和生物过程的GO注释（BP），分别有139、113和57个序列显示出对压力（SR），非生物刺激（AS）和生物刺激（BS）的响应。为了验证这一观察结果，从AS中随机选择了七个与其他物种先前研究的冷应激相关的单基因，并通过实时聚合酶链反应检查了这些基因，以了解冷和冰冻条件下表达模式的变化。强调。在计算机模拟研究中，在AS子类别中发现了138个单序列重复序列，目前正在探索其中的一些序列以评估B. rapa之间的遗传多样性。因此，unigene数据集提供了有效的资源，可用于发现与油菜抗寒和抗冻性相关的潜在基因，并且可作为芸sp属植物基因工程的有用资源。

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