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首页> 外文期刊>Israel Journal of Plant Sciences >Callus induction and plant regeneration via anther culture in mutanttomato (Lycopersicon esculentum Mill.) lines with anther abnormalities
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Callus induction and plant regeneration via anther culture in mutanttomato (Lycopersicon esculentum Mill.) lines with anther abnormalities

机译:在具有花药异常的突变番茄(Lycopersicon esculentum Mill。)品系中通过花药培养进行愈伤组织诱导和植株再生

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摘要

Anthers from two tomato mutants, solanifolia (sf) and trifoliate (tf), characterized by anther abnormalities, 4 male sterile tomato mutants (ms 15(26), ms 30, ms 32, and ins 33), and their isogenic fertile lines were cultured in vitro, using three modifications of MS media. The frequency of callus induction in the mutant anthers was significantly higher than in the wild type ones in all genotypes, a result independent of the medium. Pseudofruits surrounded by well-developed calli appeared from the anthers of San Marzano ins 15, Van's Early ins 26, San Marzano ins 30, and VF 11 Pearson ins 33. The higher callus induction in the mutant anthers correlated with higher frequency of organogenesis.
机译:来自两个番茄突变体的茄子,即茄属植物(sf)和三叶酸酯(tf),以花药异常为特征,有4个雄性不育番茄突变体(ms 15(26),ms 30,ms 32和ins 33),它们的同基因可育系是使用MS培养基的三种改良剂进行体外培养。在所有基因型中,突变型花药中愈伤组织的诱导频率均显着高于野生型。被成熟愈伤组织包围的假果出现在San Marzano ins 15,Van's Early ins 26,San Marzano ins 30和VF 11 Pearson ins 33的花药中。突变体花药中较高的愈伤组织诱导作用与较高的器官发生频率相关。

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