• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochimica et Biophysica Acta. General Subjects >Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells.
【24h】

Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells.

机译：姜黄素在正常细胞和肿瘤细胞中的定量细胞摄取，定位和细胞毒性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluorescence intensity of curcumin were significantly higher in tumor cells compared to the normal cells. A linear dependency on the uptake was observed with treatment concentration of curcumin. Using laser confocal microscopy, intracellular localization of curcumin was monitored and the results indicated that curcumin is located both in the cell membrane and the nucleus. Sub-cellular fractionation of curcumin-loaded MCF7 cells supported the differential distribution of curcumin in membrane, cytoplasm and nuclear compartments of cell with maximum localization in the membrane. Cytotoxicity studies in different cell lines indicated that the toxicity of curcumin increased with increasing uptake.
机译：使用吸收和荧光光谱法，计算了姜黄素（姜黄素的一种抗氧化剂和抗肿瘤剂）在两种类型的正常细胞中的定量细胞摄取：脾脏淋巴细胞和NIH3T3，以及两种肿瘤细胞系：EL4和MCF7。与正常细胞相比，肿瘤细胞中姜黄素的摄取和荧光强度均显着更高。在姜黄素的治疗浓度下观察到对吸收的线性依赖性。使用激光共聚焦显微镜，对姜黄素的细胞内定位进行了监测，结果表明姜黄素位于细胞膜和细胞核中。姜黄素加载的MCF7细胞的亚细胞分级分离支持姜黄素在细胞膜，细胞质和细胞核区中的差异分布，并在膜中具有最大的定位。在不同细胞系中的细胞毒性研究表明姜黄素的毒性随着摄取的增加而增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号