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首页> 外文期刊>Bulletin of Entomological Research >The effects of local selection versus dispersal on insecticide resistance patterns: longitudinal evidence from diamondback moth (Plutella xylostella (Lepidoptera: Plutellidae)) in Australia evolving resistance to pyrethroids.
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The effects of local selection versus dispersal on insecticide resistance patterns: longitudinal evidence from diamondback moth (Plutella xylostella (Lepidoptera: Plutellidae)) in Australia evolving resistance to pyrethroids.

机译:局部选择与扩散对杀虫剂抗性模式的影响:澳大利亚小菜蛾(小菜蛾(Plutella xylostella,鳞翅目:Plutellidae))对拟除虫菊酯的抗性演变的纵向证据。

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摘要

When strong directional selection acts on a trait, the spatial distribution of phenotypes may reflect effects of selection, as well as the spread of favoured genotypes by gene flow. Here we investigate the relative impact of these factors by assessing resistance to synthetic pyrethroids in a 12-year study of diamondback moth, Plutella xylostella, from southern Australia. We estimated resistance levels in populations from brassicaceous weeds, canola, forage crops and vegetables. Differences in resistance among local populations sampled repeatedly were stable over several years. Levels were lowest in samples from weeds and highest in vegetables. Resistance in canola samples increased over time as insecticide use increased. There was no evidence that selection in one area influenced resistance in adjacent areas. Microsatellite variation from 13 populations showed a low level of genetic variation among populations, with an AMOVA indicating that population only accounted for 0.25% of the molecular variation. This compared to an estimate of 13.8% of variation accounted for by the resistance trait. Results suggest that local selection rather than gene flow of resistance alleles dictated variation in resistance across populations. Therefore, regional resistance management strategies may not limit resistance evolution.
机译:当强方向选择作用于性状时,表型的空间分布可能反映选择的效果,以及受基因流影响的有利基因型的传播。在这项来自澳大利亚南部的小菜蛾蛾小菜蛾的12年研究中,我们通过评估对合成拟除虫菊酯的抗性来调查这些因素的相对影响。我们估计了芸苔属杂草,低芥酸菜子,饲料作物和蔬菜对种群的抵抗力水平。几年来,反复采样的当地居民之间的抗药性差异稳定。杂草样品中的含量最低,而蔬菜中含量最高。随着使用杀虫剂的增加,双低油菜籽样品中的抗性随时间增加。没有证据表明一个地区的选择会影响相邻地区的抵抗力。来自13个种群的微卫星变异显示出种群之间的遗传变异水平很低,而AMOVA表明种群仅占分子变异的0.25%。相比之下,抵抗性状造成的变异估计为13.8%。结果表明,抗性等位基因的局部选择而非基因流决定了群体间抗性的变化。因此,区域抵抗力管理策略可能不会限制抵抗力的发展。

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