...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemical Genetics >Assignment of Dicer gene to chicken chromosome 5 by radiation hybrid panel mapping.
【24h】

Assignment of Dicer gene to chicken chromosome 5 by radiation hybrid panel mapping.

机译：通过辐射杂交板作图将Dicer基因分配给鸡5号染色体。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RNA interference is an evolutionarily conserved gene-silencing pathway in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs (Hannon, 2002). The Dicer gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNase motif in the carboxy terminus (Matsuda et al., 2000). The encoded protein functions as a ri-bonuclease and is required by the RNA interference and small temporal RNA pathways to produce the active small RNA component that represses gene expression (Hutvagner et al., 2001; Ketting et al., 2001; Knight and Bass, 2001). In higher vertebrate cells, Dicer-related RNA interference-is involved in the formation of the heterochromatin structure. A conditional loss-of-function mutant of Dicer in chicken DT40 cells causes defects in heterochromatin formation and chromosome segregation. Depletion of Dicer results in cell death with the accumulation of abnormal mitotic cells that show premature sister chromatid separation. Aberrant accumulation of the transcript from or-satellite sequences, which consist of human centromeric repeat DNAs, was detected in Dicer-deficient cells (Fukagawa et al., 2004; White and Allshire, 2004). In spite of our knowledge of the biological function of Dicer, its functional and hereditary mechanism in chicken is not fully understood. In this report, precise localization of Dicer was first performed by RH mapping in chicken. We also compared the coding region of Dicer in different species.
机译：RNA干扰是一种进化保守的基因沉默途径，其中核酸酶Dicer将双链RNA切割成小的干扰RNA（Hannon，2002）。 Dicer基因编码一种蛋白质，该蛋白质具有RNA解旋酶基序，其氨基末端包含DEXH盒，而羧基末端具有RNase基序（Matsuda等，2000）。编码的蛋白质起ri-核糖核酸酶的作用，是RNA干扰和短暂的RNA通路所必需的，以产生抑制基因表达的活性小RNA成分（Hutvagner等，2001; Ketting等，2001; Knight和Bass ，2001）。在高级脊椎动物细胞中，Dicer相关的RNA干扰参与异染色质结构的形成。鸡DT40细胞中Dicer的条件性功能丧失突变体导致异染色质形成和染色体分离方面的缺陷。切丁机的耗尽会导致细胞死亡，并伴有异常的有丝分裂细胞的积聚，显示出过早的姐妹染色单体分离。在Dicer缺陷型细胞中检测到or卫星序列转录物的异常积累，该序列由人类着丝粒重复DNA组成（Fukagawa等，2004; White和Allshire，2004）。尽管我们了解Dicer的生物学功能，但尚未完全了解其在鸡中的功能和遗传机制。在此报告中，首先通过RH映射在鸡中对Dicer进行了精确定位。我们还比较了Dicer在不同物种中的编码区。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号