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首页> 外文期刊>Biochemical Genetics >Cloning, expression pattern, chromosomal localization, and evolution analysis of Porcine gnaq, gna11, and gna14.
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Cloning, expression pattern, chromosomal localization, and evolution analysis of Porcine gnaq, gna11, and gna14.

机译：猪gnaq，gna11和gna14的克隆，表达模式，染色体定位和进化分析。

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摘要

The Gq class is one subfamily of the G protein alpha subunits multigene family. It comprises four genes: Gnaq, Gna11, Gna14, and Gna15. In mice and humans, the alpha subunit is an essential component of G protein interaction with receptors and effectors. We report here the cloning and characterization of porcine Gnaq, Gna11, and Gna14. We have cloned the full-length coding sequences of porcine Gnaq, Gna11, and Gna14 (1,080, 1,080, and 1,068 bp, respectively) and then mapped them chromosomally to regions 1q21-27, 2q21-24, and 1q21-27 by radiation hybrid mapping. Tissue distribution analysis indicated that Gnaq and Gna11 were coexpressed in liver, heart, muscle, spleen, adipose tissue, brain, and uterus, but Gna14 mRNA was detected only in kidney and lung. The phylogenetic trees reveal that porcine Gnaq, Gna11, and Gna14 are evolutionarily closer to their human homologs. This is the first report of molecular cloning and characterization of porcine Gnaq, Gna11, and Gna14, which will be helpful for furtherunderstanding of the physiological role of Gq class genes in pigs.
机译：Gq类是G蛋白α亚基多基因家族的一个亚家族。它包含四个基因：Gnaq，Gna11，Gna14和Gna15。在小鼠和人类中，α亚基是G蛋白与受体和效应物相互作用的重要组成部分。我们在这里报告猪Gnaq，Gna11和Gna14的克隆和鉴定。我们已经克隆了猪Gnaq，Gna11和Gna14的全长编码序列（分别为1,080、1,080和1,068 bp），然后通过辐射杂交将它们染色体映射到区域1q21-27、2q21-24和1q21-27。映射。组织分布分析表明，Gnaq和Gna11在肝，心脏，肌肉，脾脏，脂肪组织，脑和子宫中共表达，但仅在肾和肺中检测到Gna14 mRNA。系统进化树显示，猪Gnaq，Gna11和Gna14在进化上更接近于人类同源物。这是关于猪Gnaq，Gna11和Gna14分子克隆和鉴定的首次报道，这将有助于进一步了解Gq类基因在猪中的生理作用。

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