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首页> 外文期刊>Biochemical Genetics >Evidence of sibling species in the brown planthopper complex (Nilaparvata lugens) detected from short and long primer random amplified polymorphic DNA fingerprints.
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Evidence of sibling species in the brown planthopper complex (Nilaparvata lugens) detected from short and long primer random amplified polymorphic DNA fingerprints.

机译：从短和长引物随机扩增的多态性DNA指纹图中检测到棕色飞虱复合体（Nilaparvata lugens）中有同胞物种的证据。

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摘要

The inheritance of 31 amplicons from short and long primer RAPD was tested for segregating ratios in two families of the brown planthopper, Nilaparvata lugens, and they were found to be inherited in a simple Mendelian fashion. These markers could now be used in population genetics studies of N. lugens. Ten populations of N. lugens were collected from five locations in Malaysia. Each location had two sympatric populations. Cluster and principal coordinate analyses based on genetic distance along with AMOVA revealed that the rice-infesting populations (with high esterase activity) at five localities clustered together as a group, and Leersia-infesting populations (with low esterase activity) at the same localities formed another distinct cluster. Two amplicons from primers OPD03 (0.65 kb) and peh#6 (1.0 kb) could be considered diagnostic bands, which were fixed in the Leersia-infesting populations. These results represent evidence of a sibling species in the N. lugens complex.
机译：测试了来自短和长引物RAPD的31个扩增子的遗传在两个褐飞虱科（Nilaparvata lugens）中的分离比例，发现它们以简单的孟德尔遗传方式遗传。这些标记现在可用于黄褐猪笼草的种群遗传学研究。从马来西亚的五个地方收集了十个发光猪笼草种群。每个地点有两个同胞人口。基于遗传距离和AMOVA的聚类和主坐标分析显示，五个地区的水稻害虫种群（具有高酯酶活性）聚集在一起，而同一地区的水稻害虫种群（具有低酯酶活性）形成了群体另一个独特的集群。来自引物OPD03（0.65 kb）和peh＃6（1.0 kb）的两个扩增子可被认为是诊断条带，它们固定在感染莱氏菌的人群中。这些结果代表了N. lugens复合物中同胞物种的证据。

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