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首页> 外文期刊>Biochemical Genetics >Sequence characterization, polymorphism, and chromosomal localizations of the porcine CapZ genes.
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Sequence characterization, polymorphism, and chromosomal localizations of the porcine CapZ genes.

机译：猪CapZ基因的序列表征，多态性和染色体定位。

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摘要

CapZ is a widely distributed and highly conserved actin-binding protein that caps the barbed end of actin filaments and nucleates actin polymerization in a Ca(2+)-independent manner. In myofibrils, it is localized in the Z-lines. In this study, we cloned and characterized Capz subunit genes from the pig muscle. The nucleotide sequences and the predicted protein sequences share high sequence identity with other mammalian orthologs. The reverse transcriptase polymerase chain reaction (RT-PCR) revealed that porcine Capzbeta, Capzalpha1, and Capzalpha2 genes are expressed in all 11 tissues studied (liver, spleen, small intestine, large intestine, lymph node, kidney, heart, skeletal muscle, brain, fat, and lung) but in variable amounts. Radiation hybrid mapping data indicated that Capzbeta, Capzalpha1, and Capzalpha2 map to q2.1-2.6 of pig chromosome 6 (SSC6), q1.6-q2.2 of pig chromosome 4 (SSC4), and q1.3-2.3 of pig chromosome 18 (SSC18), respectively. An A/C single nucleotide polymorphism in Capzbeta intron 4 was identified with a HhaI PCR restriction fragment length polymorphism, which showed great allele frequency differences between Guizhou Xiang, Guangxi Bama, Wuzhishan, Tongcheng, Landrace, and Yorkshire pigs. The association analysis suggested that the Capzbeta genotype was associated with leaf fat (P < 0.05) in our experimental population.
机译：CapZ是一种分布广泛且高度保守的肌动蛋白结合蛋白，可覆盖肌动蛋白丝的倒刺末端并以独立于Ca（2+）的方式使肌动蛋白聚合成核。在肌原纤维中，它位于Z线。在这项研究中，我们从猪肌肉中克隆并鉴定了Capz亚基基因。核苷酸序列和预测的蛋白质序列与其他哺乳动物直系同源物具有高度的序列同一性。逆转录聚合酶链反应（RT-PCR）显示，猪Capzbeta，Capzalpha1和Capzalpha2基因在所有研究的11种组织（肝，脾，小肠，大肠，淋巴结，肾脏，心脏，骨骼肌，脑）中表达，脂肪和肺），但数量可变。辐射混合作图数据表明Capzbeta，Capzalpha1和Capzalpha2分别映射到猪6号染色体（SSC6）的q2.1-2.6，猪4号染色体（SSC4）的q1.6-q2.2和猪的q1.3-2.3第18号染色体（SSC18）。通过HhaI PCR限制性片段长度多态性鉴定Capzbeta内含子4中的A / C单核苷酸多态性，这表明贵州香猪，广西巴马，五指山，通城，长白猪和约克郡猪之间存在巨大的等位基因频率差异。关联分析表明，在我们的实验人群中，Capzbeta基因型与叶脂肪相关（P <0.05）。

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