...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Ecotoxicology >Establishment of toxicity thresholds in subpopulations of coelomocytes (amoebocytes vs. eleocytes) of Eisenia fetida exposed in vitro to a variety of metals: implications for biomarker measurements
【24h】

Establishment of toxicity thresholds in subpopulations of coelomocytes (amoebocytes vs. eleocytes) of Eisenia fetida exposed in vitro to a variety of metals: implications for biomarker measurements

机译：建立在体外暴露于多种金属的费兹艾氏体腔细胞的亚群（变形细胞相对于细胞）的毒性阈值：对生物标志物测量的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Coelomocytes comprise the immune system of earthworms and due to their sensitivity responding to a wide range of pollutants have been widely used as target cells in soil ecotoxicology. Recently, in vitro assays with primary cultures of coelomocytes based in the neutral red uptake (NRU) assay have been developed as promising tools for toxicity assessment chemical in a reproducible and cost-effective manner. However, NRU showed a bimodal dose-response curve previously described after in vivo and in vitro exposure of earthworm coelomocytes to pollutants. This response could be related with alterations in the relative proportion of coelomocyte subpopulations, amoebocytes and eleocytes. Thus, the aims of the present work were, first, to establish the toxicity thresholds that could be governed by different cell-specific sensitivities of coelomocytes subpopulations against a series of metals (Cu, Cd, Pb, Ni), and second to understand the implication that coelomocyte population dynamics (eleocytes vs. amoebocytes) after exposure to pollutants can have on the viability of coelomocytes (measured by NRU assay) as biomarker of general stress in soil health assessment. Complementarily flow cytometric analyses were applied to obtain correlative information about single cells (amoebocytes and eleocytes) in terms of size and complexity, changes in their relative proportion and mortality rates. The results indicated a clear difference in sensitivity of eleocytes and amoebocytes against metal exposure, being eleocytes more sensitive. The bimodal dose-response curve of NRU after in vitro exposure of primary cultures of coelomocytes to metals revealed an initial mortality of eleocytes (decreased NRU), followed by an increased complexity of amoebocytes (enhanced phagocytosis) and massive mortality of eleocytes (increased NRU), to give raise to a massive mortality of amoebocytes (decrease NRU). A synergistic effect on NRU was exerted by the exposure to high Cu concentrations and acidic pH (elicited by the metal itself), whereas the effects on NRU produced after exposure to Cd, Ni and Pb were due solely to the presence of metals, being the acidification of culture medium meaningless.
机译：体细胞是comprise的免疫系统，由于它们对多种污染物的反应敏感，已被广泛用作土壤生态毒理学中的靶细胞。近来，已经开发了基于中性红吸收（NRU）测定法的以内皮细胞的原代培养物进行的体外测定法，作为以可再现且经济有效的方式进行毒性评估的有前途的工具。然而，NRU在co体内的体外和体外暴露于污染物后显示了先前描述的双峰剂量反应曲线。这种反应可能与结肠内皮细胞亚群，变形细胞和单核细胞相对比例的改变有关。因此，本研究的目的首先是确定毒性阈值，该阈值可以通过对一系列金属（Cu，Cd，Pb，Ni）对结肠细胞亚群的不同细胞特异性敏感性进行控制，其次要了解这暗示着暴露于污染物后的白细胞数量动态（电单细胞与变形细胞）可能会影响白细胞的活力（通过NRU测定法），作为土壤健康评估中一般胁迫的生物标记。应用互补流式细胞仪分析获得有关单个细胞（变形细胞和单核细胞）的相关信息，包括大小和复杂性，相对比例的变化和死亡率。结果表明，细胞和金属变形细胞对金属暴露的敏感性存在明显差异，因为细胞更敏感。 NRU的原代培养物体外暴露于金属后，NRU的双峰剂量反应曲线表明，最初的细胞死亡（NRU减少），随后是变形细胞的复杂性增加（吞噬作用增强）和大量的死亡（NRU增加） ，导致大量成虫细胞死亡（降低NRU）。暴露于高浓度的铜和酸性pH（由金属本身引起）对NRU产生协同作用，而暴露于Cd，Ni和Pb后产生的对NRU的影响仅归因于金属的存在，即培养基酸化毫无意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号