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首页> 外文期刊>Biochemical Genetics >Characterization and SNP identification of part of the goat melanophilin gene.
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Characterization and SNP identification of part of the goat melanophilin gene.

机译：山羊嗜黑素蛋白基因部分特征和SNP鉴定。

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摘要

The melanophilin (MLPH) gene has been characterized as the candidate gene for dilute coat color in some species, but little is known about it in the goat. In this study, part of the genomic DNA sequence (19,289 bp) containing the whole coding region of the MLPH gene from goat, as well as from sheep, was determined. We found 16 exons and 15 introns; the coding region was 1767 bp distributed in 15 exons (2-16). In sheep, the length of part of the genomic DNA sequence was 16,988 bp, with 16 exons and 15 introns, and the coding region was 1833 bp, distributed in 15 exons (2-16). Dozens of SNPs as well as some noticeable motifs in the goat MLPH gene were found during the process of sequencing and polymorphism screening. Based on the SSR Tool, three simple sequence repeat motifs were detected in the goat and sheep DNA sequences. Compared with cattle, we found insertions of 4 amino acids in goats and 26 amino acids in sheep.
机译：在某些物种中，黑色素蛋白（MLPH）基因已被表征为淡化毛色的候选基因，但在山羊中对其知之甚少。在这项研究中，确定了包含来自山羊以及来自绵羊的MLPH基因的整个编码区的部分基因组DNA序列（19,289 bp）。我们发现了16个外显子和15个内含子。编码区为1767 bp，分布在15个外显子中（2-16）。在绵羊中，基因组DNA序列的部分长度为16,988 bp，具有16个外显子和15个内含子，编码区为1833 bp，分布在15个外显子中（2-16）。在测序和多态性筛选的过程中，发现了数十个SNP以及山羊MLPH基因中的一些显着基序。基于SSR工具，在山羊和绵羊DNA序列中检测到三个简单的序列重复基序。与牛相比，我们发现山羊中插入了4个氨基酸，绵羊中插入了26个氨基酸。

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