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首页> 外文期刊>Biochemical Genetics >Molecular characterization of the full-length coding sequence of the caprine laminin receptor gene (RPSA).
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Molecular characterization of the full-length coding sequence of the caprine laminin receptor gene (RPSA).

机译:山羊层粘连蛋白受体基因(RPSA)的全长编码序列的分子表征。

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摘要

Scrapie is a prion disease in sheep and goats. Ribosomal protein SA (RPSA), also called 37 kDa laminin receptor precursor/67 kDa laminin receptor has been demonstrated to be a putative cell surface receptor for prion. To investigate the caprine RPSA, we cloned the full-length coding sequence of the gene of goat and submitted it to GenBank. The length of the open reading frame is 888 bp, encoding 295 amino acids. The putative amino acid sequence is highly similar to that of other mammals. The caprine amino acid sequence of RPSA is shown to be identical to the sequence of species susceptible to scrapie at positions 241, 272, and 291. The phylogenetic tree analysis revealed that the genetic distance between sheep and goat is the smallest. Moreover, RT-PCR results of 11 tissues indicated that RPSA mRNA is expressed in all selected caprine tissues.
机译:rap痒病是绵羊和山羊的a病毒病。核糖体蛋白SA(RPSA),也称为37 kDa层粘连蛋白受体前体/ 67 kDa层粘连蛋白受体,已被证明是病毒的假定细胞表面受体。为了研究鼠RPSA,我们克隆了山羊基因的全长编码序列,并将其提交给GenBank。开放阅读框的长度是888bp,编码295个氨基酸。推定的氨基酸序列与其他哺乳动物的序列高度相似。 RPSA的鼠科氨基酸序列显示与在241、272和291位易患瘙痒病的物种序列相同。系统树分析表明,绵羊和山羊之间的遗传距离最小。此外,11个组织的RT-PCR结果表明RPSA mRNA在所有选择的山羊组织中都有表达。

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