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首页> 外文期刊>Biochemical Genetics >Isolation of 106 microsatellite markers for the pacific abalone from a trinucleotide-enriched library.
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Isolation of 106 microsatellite markers for the pacific abalone from a trinucleotide-enriched library.

机译：从富含三核苷酸的文库中分离出106个用于太平洋鲍鱼的微卫星标记。

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摘要

The Pacific abalone, Haliotis discus hannai Ino, has been considered one of the most flavorful species among molluscs in many Asian countries (Sekino and Hara 2001). Numerous genetic breeding programs have been initiated to improve its economically important traits, such as growth rate and disease resistance. Several of these traits are controlled primarily by quantitative trait loci (QTL), the genetic bases of which are studied using high-density linkage maps. Microsatellites are one of the most popular types of markers for constructing genetic linkage maps because of their codominant inheritance, high degree of polymorphism, large volume of information on segregation, and transferability among mapping families (Tautz 1989; Toth et al. 2000; Zhan et al. 2009). The majority of microsatellite markers developed for the Pacific abalone are dinucleotides (Li et al. 2002; An and Han 2006; Sun et al. 2007; Zhan et al. 2008; Li et al. 2010). The trinucleotide microsatellites can be more easily and accurately scored, however, because fewer ghost bands/peaks are associated with them than with the dinucleotides (e.g., Rongwen et al. 1995). The objectives for our researches were to increase the diversification of microsatellite markers and to construct a high-density linkage map for the Pacific abalone, and we succeeded in isolating 106 novel polymorphic trinucleotide microsatellite markers.
机译：太平洋鲍鱼Haliotis discus hannai Ino被认为是许多亚洲国家软体动物中味道最浓的物种之一（Sekino and Hara 2001）。已经启动了许多遗传育种计划，以改善其经济上重要的性状，例如生长速度和抗病性。其中一些性状主要由数量性状基因座（QTL）控制，其遗传基础使用高密度连锁图谱进行研究。微卫星是遗传连锁图谱构建中最流行的标记类型之一，因为它们具有显着的遗传性，高度的多态性，大量的分离信息以及在图谱族之间的可转移性（Tautz 1989; Toth等人2000; Zhan等人）。 （2009年）。为太平洋鲍鱼开发的大多数微卫星标记是二核苷酸（Li等，2002; An和Han，2006; Sun等，2007; Zhan等，2008; Li等，2010）。然而，三核苷酸微卫星可以更容易和准确地计分，因为与二核苷酸相比，与它们相关的鬼带/峰更少（例如，Rongwen等人，1995）。我们的研究目标是增加微卫星标记的多样性，并为太平洋鲍鱼构建高密度连锁图谱，我们成功地分离了106种新颖的多态三核苷酸微卫星标记。

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