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首页> 外文期刊>Insect Biochemistry and Molecular Biology >y Agonist-mediated activation of Bombyx mori diapause hormone receptor signals to extracellular signal-regulated kinases 1 and 2 through Gq-PLC-PKC-dependent cascade
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y Agonist-mediated activation of Bombyx mori diapause hormone receptor signals to extracellular signal-regulated kinases 1 and 2 through Gq-PLC-PKC-dependent cascade

机译：γ激动剂介导的家蚕滞育激素受体信号通过依赖Gq-PLC-PKC的级联反应激活至细胞外信号调节激酶1和2

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摘要

Diapause is a developmental strategy adopted by insects to survive in challenging environments such as the low temperatures of a winter. This unique process is regulated by diapause hormone (DH), which is a neuropeptide hormone that induces egg diapause in Bombyx mori and is involved in terminating pupal diapause in heliothis moths. An G protein-coupled receptor from the silkworm, B. mori, has been identified as a specific cell surface receptor for DH. However, the detailed information on the DH-DHR system and its mechanism(s) involved in the induction of embryonic diapause remains unknown. Here, we combined functional assays with various specific inhibitors to elucidate the DHR-mediated signaling pathways. Upon activation by DH, B. mori DHR is coupled to the Gq protein, leading to a significant increase of intracellular Ca2+ and cAMP response element-driven luciferase activity in an UBO-QIC, a specific Gq inhibitor, sensitive manner. B. mon DHR elicited ERK1/2 phosphorylation in a dose- and time-dependent manner in response to DH. This effect was almost completely inhibited by co-incubation with UBO-QIC and was also significantly suppressed by PLC inhibitor U73122, PKC inhibitors Gd6983 and the Ca2+ chelator EGTA. Moreover, DHR-induced activation of ERK1/2 was significantly attenuated by treatment with the G beta gamma specific inhibitors gallein and M119K and the PI3K specific inhibitor Wortmannin, but not by the Src specific inhibitor PP2. Our data also demonstrates that the EGFR-transactivation pathway is not involved in the DHR-mediated ERK1/2 phosphorylation. Future efforts are needed to clarify the role of the ERK1/2 signaling pathway in the DH-mediated induction of B. mori embryonic diapause. (C) 2016 Elsevier Ltd. All rights reserved.
机译：滞育是昆虫采用的发育策略，可以在严峻的环境（例如冬天的低温）中生存。这个独特的过程受滞育激素（DH）调节，后者是一种神经肽激素，可在家蚕中引起卵滞育，并参与终止天蛾蛾的幼仔滞育。来自家蚕的G蛋白偶联受体，已被鉴定为DH的特定细胞表面受体。然而，关于DH-DHR系统及其参与诱导胚胎滞育的机制的详细信息仍是未知的。在这里，我们将功能测定与各种特定的抑制剂相结合，以阐明DHR介导的信号通路。在被DH激活后，家蚕BHR与Gq蛋白偶联，以UBO-QIC（一种特异性的Gq抑制剂）敏感的方式导致细胞内Ca2 +和cAMP响应元件驱动的荧光素酶活性显着增加。 B. mon DHR响应DH以剂量和时间依赖性方式引起ERK1 / 2磷酸化。与UBO-QIC共同孵育几乎完全抑制了这种作用，PLC抑制剂U73122，PKC抑制剂Gd6983和Ca2 +螯合剂EGTA也显着抑制了这种作用。此外，通过使用G betaγ特异性抑制剂gallein和M119K和PI3K特异性抑制剂Wortmannin治疗，DHR诱导的ERK1 / 2活化显着减弱，而不受Src特异性抑制剂PP2治疗。我们的数据还证明，EGFR转激活途径不参与DHR介导的ERK1 / 2磷酸化。需要进一步的努力来阐明ERK1 / 2信号通路在DH介导的桑蚕芽孢杆菌滞育中的作用。 （C）2016 Elsevier Ltd.保留所有权利。

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