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首页> 外文期刊>Biochemistry and molecular biology education >Use of a Laboratory Exercise on Molar Absorptivity to Help Students Understand the Authority of the Primary Literature
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Use of a Laboratory Exercise on Molar Absorptivity to Help Students Understand the Authority of the Primary Literature

机译：使用一项关于磨牙吸收性的实验室练习来帮助学生了解主要文献的权威

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摘要

To promote understanding of the authority of the primary literature in students taking our biochemistry laboratory courses, a biochemistry laboratory exercise on the determination of an acceptable molar absorptivity value of 2-nitrophenol (2-NP) was developed. This made the laboratory course much more relevant by linking to a thematic thread, β-galactosidase, that scaffolds concepts in one exercise with those in later exercises. The substrate for the continuous assay of β-galactosidase is the chromogenic 2-nitrophenyl-β-D-galactopyranoside that produces 2-NP. In an early laboratory exercise, students determine the wavelength of maximum absorption for the protonated and deprotonated form of 2-NP at various pH values and then determine the molar absorptivity of 2-NP. Students were encouraged to discuss apparent discrepancies not only in their own determinations of molar absorptivity values for 2-NP, but also in the published molar absorptivity values for 2-NP (2,150-21,300 M~(-1) cm~(-1)) at almost the same pH and at 420 nm. Finally, the students were led to a publication that serves as an authentic source for molar absorptivity of 2-NP.
机译：为了提高对参加我们生物化学实验室课程的学生的主要文献权威的理解，开发了一种生物化学实验室练习，用于确定2-硝基苯酚（2-NP）的可接受的摩尔吸收率值。通过链接到主题线程β-半乳糖苷酶，可以使实验室课程更加相关，该主题线程可在一次练习中支撑概念，在以后的练习中支撑概念。连续测定β-半乳糖苷酶的底物是产生2-NP的发色2-​​硝基苯基-β-D-吡喃半乳糖苷。在早期的实验室练习中，学生可以确定各种pH值下2-NP的质子化和去质子化形式的最大吸收波长，然后确定2-NP的摩尔吸收率。鼓励学生不仅在自己确定2-NP的摩尔吸收率值时讨论明显的差异，而且在已发表的2-NP（2,150-21,300 M〜（-1）cm〜（-1）的摩尔吸收率值中，讨论明显的差异。 ）在几乎相同的pH值和420 nm下。最终，学生们被带到了出版物中，该出版物可作为2-NP摩尔吸收率的可靠来源。

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