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Gene expression profiles among immature and adult reproductive castes of the termite Reticulitermes flavipes

机译:白蚁网状黄体的未成熟和成年生殖种姓的基因表达谱

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Array-based genomic studies were conducted with the goal of identifying immature (i.e. nymph) and adult reproductive caste-biased gene expression in the termite Reticulitermes flavipes. Using cDNA macro-arrays, we identified thirty-four nymph-biased genes falling into eight ontogenic categories. Based on gene expression profiles among diverse castes and developmental stages (determined by quantitative PCR), several important trends emerged. These findings highlight the importance of several developmental and survival-based factors among immature and adult termite reproductives, including: vitellogenesis, nutrient storage, juvenile hormone sequestration, ribosomal translational and filtering mechanisms, fatty acid biosynthesis, apoptosis inhibition, and both endogenous and symbiont cellulase-assisted nutrition. These findings are highly significant as they are the first to elucidate the molecular biology underlying termite reproductive caste differentiation and reproductive caste-specific biology. Other gene expression results are in agreement with previous findings that suggest roles for vitellogenin-like haemolymph proteins in soldier caste differentiation.
机译:进行基于阵列的基因组研究的目的是鉴定白蚁网纹黄fl中的未成熟(即若虫)和成年生殖种姓偏向的基因表达。使用cDNA宏阵列,我们确定了34个若虫偏向的基因,它们属于8个本体基因类别。基于不同种姓和发育阶段(通过定量PCR确定)之间的基因表达谱,出现了一些重要趋势。这些发现突显了未成熟和成年白蚁繁殖中几个以发育和生存为基础的因素的重要性,包括:卵黄发生,营养物储存,少年激素隔离,核糖体翻译和过滤机制,脂肪酸生物合成,细胞凋亡抑制以及内源和共生纤维素酶辅助营养。这些发现非常有意义,因为它们是第一个阐明白蚁生殖种姓分化和生殖种姓特异性生物学基础的分子生物学。其他基因表达结果与以前的发现相吻合,这些发现表明卵黄蛋白原样血淋巴蛋白在士兵种姓分化中的作用。

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