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首页> 外文期刊>Insect Molecular Biology >Characterization of the Drosophila melanogaster retinin gene encoding a cornea-specific protein
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Characterization of the Drosophila melanogaster retinin gene encoding a cornea-specific protein

机译：果蝇黑角膜视黄素编码角膜特异性蛋白的基因的表征。

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摘要

Two-dimensional analysis of head extracts from Drosophila melanogaster identified the four eye-specific protein spots corresponding to the retinin protein. The retinin protein spots were specifically stained with phosphoprotein-specific dye, suggesting that the retinin protein undergoes post-translational modification by phosphorylation. Northern blot analysis showed that the retinin gene begins to be expressed during the late stage of puparium formation during development. Analysis of the N-terminal sequence and expression of the retinin gene in S2 suggest that retinin is a secretory protein. Transgenic flies with knockdown expression of the retinin gene by RNA interference (RNAi) were established. However, no significant phenotypic changes in eye structure or phototransduction were observed in the transgenic flies. Western blot analysis and immunohistochemical studies of D. melanogaster eyes suggest that retinin is a cornea-specific protein.
机译：果蝇果头提取物的二维分析确定了与视黄素蛋白相对应的四个眼特异性蛋白斑点。视黄素蛋白斑点用磷蛋白特异性染料特异性染色，表明视黄素蛋白通过磷酸化进行翻译后修饰。 Northern印迹分析表明，视黄素基因在发育过程中的par形成后期开始表达。对S2中N端序列和视黄素基因表达的分析表明，视黄素是一种分泌蛋白。建立了通过RNA干扰（RNAi）抑制视黄素基因表达的转基因果蝇。然而，在转基因果蝇中没有观察到眼睛结构或光转导的显着表型变化。 Western blot分析和黑腹果蝇眼睛的免疫组织化学研究表明，视黄素是一种角膜特异性蛋白。

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