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首页> 外文期刊>Insect Molecular Biology >Molecular cloning and characterization of cDNAs encoding dopamine receptor-1 and -2 from brain-suboesophageal ganglion of the silkworm, Bombyx mori
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Molecular cloning and characterization of cDNAs encoding dopamine receptor-1 and -2 from brain-suboesophageal ganglion of the silkworm, Bombyx mori

机译：家蚕脑-食管下神经节编码多巴胺受体-1和-2的cDNA的分子克隆与鉴定

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摘要

In order to better understand the relationship between dopamine and the release of diapause hormone into the blood, we cloned and characterized cDNAs encoding Bombyx mori dopamine receptor-1 and -2 (BmDopR1 and 2) from the pupal brain-suboesophageal ganglion. BmDopR1 and 2 had high similarities to group 1 (Drosophila melanogaster DOP1 and Apis mellifera DOP1) and group 2 (D. melanogaster DopR99B, A. mellifera DOP2 and Papilio xuthus DOP1), respectively. When BmDopR1 and 2 were expressed in human embryonic kidney (HEK) cells, they responded to dopamine by increasing intracellular cAMP levels, thus indicating the presence of D1-like receptors. There were no clear differences in BmDopR1 and 2 mRNA levels between brain-suboesophageal ganglion complexes of diapause and nondiapause egg producers during pupal-adult development. BmDopR1 and 2 mRNAs were concentrated in the mushroom body calyx rather than in the suboesophageal ganglion. Taking into account the results of earlier experiments on excised regions corresponding to mushroom bodies, BmDopR1 and 2 in the mushroom body apparently play a role in the release of diapause hormone.
机译：为了更好地了解多巴胺与滞育激素向血液中的释放之间的关系，我们从and脑-食管下神经节克隆并鉴定了编码家蚕多巴胺受体-1和-2（BmDopR1和2）的cDNA。 BmDopR1和2分别与第1组（黑腹果蝇DOP1和蜜蜂Api mellifera DOP1）和第2组（黑腹果蝇DopR99B，A。mellifera DOP2和Papilio xuthus DOP1）具有高度相似性。当BmDopR1和2在人类胚胎肾脏（HEK）细胞中表达时，它们通过增加细胞内cAMP水平来响应多巴胺，从而表明存在D1样受体。在成年up发育过程中，滞育和非滞育产卵者的脑-食管下神经节复合体之间的BmDopR1和2 mRNA水平没有明显差异。 BmDopR1和2 mRNAs集中在蘑菇体萼，而不是在食管下神经节。考虑到较早的对与蘑菇体相对应的切除区域的实验结果，蘑菇体内的BmDopR1和2显然在滞育激素的释放中起作用。

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