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首页> 外文期刊>Insect Molecular Biology >Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes
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Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes

机译：鉴定和鉴定了编码Nephila锁骨的主要壶腹丝蛋白的多个Spidroin 1基因

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摘要

Spider dragline silk is primarily composed of proteins called major ampullate spidroins (MaSps) that consist of a large repeat array flanked by nonrepetitive N- and C-terminal domains. Until recently, there has been little evidence for more than one gene encoding each of the two major spidroin silk proteins, MaSp1 and MaSp2. Here, we report the deduced N-terminal domain sequences for two distinct MaSp1 genes from Nephila clavipes (MaSp1A and MaSp1B) and for MaSp2. All three MaSp genes are co-expressed in the major ampullate gland. A search of the GenBank database also revealed two distinct MaSp1 C-terminal domain sequences. Sequencing confirmed that both MaSp1 genes are present in all seven Nephila clavipes spiders examined. The presence of nucleotide polymorphisms in these genes confirmed that MaSp1A and MaSp1B are distinct genetic loci and not merely alleles of the same gene. We experimentally determined the transcription start sites for all three MaSp genes and established preliminary pairing between the two MaSp1 N- and C-terminal domains. Phylogenetic analysis of these new sequences and other published MaSp N- and C-terminal domain sequences illustrated that duplications of MaSp genes may be widespread among spider species.
机译：蜘蛛拉丝植物丝主要由称为主要壶腹spidroins（MaSps）的蛋白质组成，该蛋白质由侧接非重复性N和C末端结构域的大型重复序列组成。直到最近，几乎没有证据表明有一个以上的基因编码两种主要的spidroin丝蛋白，MaSp1和MaSp2。在这里，我们报告的推导的N末端域序列的两个不同的MaSp1基因从锁骨线虫（MaSp1A和MaSp1B）和MaSp2。这三个MaSp基因在主要壶腹腺中共表达。 GenBank数据库的搜索还显示了两个不同的MaSp1 C末端域序列。测序证实，这两个MaSp1基因均存在于所检查的所有七只Nephila锁骨蜘蛛中。这些基因中核苷酸多态性的存在证实，MaSp1A和MaSp1B是不同的遗传基因座，而不仅仅是同一基因的等位基因。我们实验确定了所有三个MaSp基因的转录起始位点，并在两个MaSp1 N和C末端结构域之间建立了初步的配对。对这些新序列以及其他已发表的MaSp N和C末端结构域序列进行的系统进化分析表明，MaSp基因的重复可能在蜘蛛物种中广泛存在。

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