...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Insect Molecular Biology >PsOr1, a potential target for RNA interference-based pest management
【24h】

PsOr1, a potential target for RNA interference-based pest management

机译：PsOr1，基于RNA干扰的有害生物管理的潜在目标

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Insect pests cause billions of dollars in agricultural losses, and attempts to kill them have resulted in growing threats from insecticide resistance, dietary pesticide pollution and environmental destruction. New approaches to control refractory insect pests are therefore needed. The host-plant preferences of insect pests rely on olfaction and are mediated via a seven transmembrane-domain odorant receptor (Or) family. The present study reports the cloning and characterization of PsOr1, the first candidate member of the Or gene family from Phyllotreta striolata, a devastating beetle pest that causes damage worldwide. PsOr1 is remarkably well conserved with respect to other insect orthologues, including DmOr83b from Drosophila melanogaster. These insect orthologues form an essential non-conventional Or sub-family and may play an important and generalized role in insect olfaction. We designed double-stranded (ds) RNA directly against the PsOr1 gene and exploited RNA interference (RNAi) to control P. striolata. The chemotactic behavioural measurements showed that adult beetles were unable to sense the attractant or repellent odour stimulus after microinjection of dsRNA against PsOr1. Reverse Transcription (RT)-PCR analysis showed specific down-regulation of mRNA transcript levels for this gene. Furthermore, host-plant preference experiments confirmed that silencing PsOr1 by RNAi treatment impaired the host-plant preferences of P. striolata for cruciferous vegetables. These results demonstrate that this insect control approach of using RNAi to target PsOr1 and its orthologues might be effective in blocking host-plant-seeking behaviours in diverse insect pests. The results also support the theory that this unique receptor type plays an essential general role in insect olfaction.
机译：虫害在农业上造成数十亿美元的损失，而杀灭它们的企图导致了对杀虫剂的抵抗，饮食中的农药污染和环境破坏等日益严重的威胁。因此需要控制难治性害虫的新方法。害虫对宿主植物的偏好取决于嗅觉，并通过七个跨膜结构的气味受体（Or）家族介导。本研究报告了PsOr1的克隆和鉴定，PsOr1是Phyllotreta striolata的Or基因家族的第一个候选成员，Phyllotreta striolata是一种破坏性的甲虫，在世界范围内引起破坏。 PsOr1与其他昆虫直系同源物，包括来自果蝇的DmOr83b相比，具有非常好的保守性。这些昆虫直系同源物形成基本的非常规或亚科，并且可能在昆虫嗅觉中起重要而普遍的作用。我们直接针对PsOr1基因设计了双链（ds）RNA，并利用RNA干扰（RNAi）来控制条纹状疟原虫。趋化行为测量表明，成年甲虫在针对PsOr1的dsRNA显微注射后无法感知引诱剂或驱避剂的气味刺激。逆转录（RT）-PCR分析显示该基因的mRNA转录水平有特定的下调。此外，寄主植物偏好实验证实，通过RNAi处理使PsOr1沉默会损害十字花科对十字花科蔬菜的寄主植物偏好。这些结果表明，使用RNAi靶向PsOr1及其直系同源物的这种昆虫控制方法可能有效地阻止了多种害虫中寻找宿主植物的行为。结果也支持这种独特的受体类型在昆虫嗅觉中起着至关重要的一般作用的理论。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号