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首页> 外文期刊>Insect Molecular Biology >Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster
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Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster

机译：Wolbachia基因对黑腹果蝇细胞质不相容性表达影响的功能测试

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Wolbachia are inherited intracellular bacteria that infect a broad range of invertebrate hosts. They commonly manipulate host reproduction in a variety of ways and thereby favour their invasion into host populations. While the biology of Wolbachia has been extensively studied at the ecological and phenotypic level, little is known about the molecular mechanisms underlying the interaction between Wolbachia and their hosts. Recent comparative genomics studies of Wolbachia strains have revealed putative candidate genes involved in the expression of cytoplasmic incompatibility (CI) in insects. However the functional testing of these genes is hindered by the lack of available genetic tools in Wolbachia. To circumvent this problem we generated transgenic Drosophila lines expressing various Wolbachia CI candidate genes under the control of the GAL4/UAS system in order to evaluate their possible role in Wolbachia-related phenotypes in Drosophila. The expression of a number of these genes in Drosophila melanogaster failed to mimic or alter CI phenotypes across a range of Wolbachia backgrounds or in the absence of Wolbachia.
机译：沃尔巴氏菌是遗传性细胞内细菌，可感染多种无脊椎动物宿主。它们通常以多种方式操纵宿主繁殖，从而有利于其侵入宿主种群。尽管已经在生态学和表型水平上对沃尔巴氏菌的生物学进行了广泛的研究，但对沃尔巴氏菌与其宿主之间相互作用的分子机制知之甚少。 Wolbachia菌株的最新比较基因组学研究表明，推测的候选基因参与了昆虫细胞质不相容性（CI）的表达。但是，这些基因的功能测试受到沃尔巴克氏菌缺乏可用遗传工具的阻碍。为了解决这个问题，我们在GAL4 / UAS系统的控制下生成了表达各种Wolbachia CI候选基因的转基因果蝇品系，以评估它们在果蝇中与Wolbachia相关表型的可能作用。果蝇中的许多这些基因的表达无法模拟或改变整个Wolbachia背景或没有Wolbachia的CI表型。

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