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首页> 外文期刊>Insect Molecular Biology >Development of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae). (Special Issue: The Nasonia genome.)
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Development of bacterial artificial chromosome library resources for parasitoid Hymenoptera (Nasonia vitripennis and Nasonia giraulti: Pteromalidae). (Special Issue: The Nasonia genome.)

机译:开发拟寄生虫膜翅目( Vistripennis 和 Nasonia giraulti :翼缘科)的细菌人工染色体文库资源。 (特刊:Nasonia基因组。)

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摘要

The species of the genus Nasonia possess qualities that make them excellent candidates for genetic and genomic studies. To increase the wealth of genomic resources for the genus we constructed publicly available bacterial artificial chromosome (BAC) libraries for Nasonia vitripennis and Nasonia giraulti. Libraries have 36 864 clones each, empty-vector contents of ~2% and average insert sizes of 113.1 and 97.7 Kb, respectively, representing 12 and 11 genome equivalents. The N. vitripennis library was used for genome sequence assembly and in efforts at positional cloning of a developmental gene. The genome assembly of N. vitripennis is currently composed on 6181 un-joined scaffolds. These BAC libraries can be used to identify and close regions between scaffolds of the genome assemblies of both species.
机译:纳斯尼亚属(Nasonia)的种类具有使其成为遗传和基因组研究的极佳候选者的特质。为了增加该属的基因组资源,我们为 vitripennis 和 gisault giaulti 建立了可公开获得的细菌人工染色体(BAC)库。图书馆各有36 864个克隆,空载体含量约为2%,平均插入片段大小分别为113.1和97.7 Kb,分别代表12和11个基因组当量。 N。 vitripennis 文库用于基因组序列组装和努力定位发育基因的位置克隆。 N的基因组组装。 vitripennis 目前由6181个未连接的脚手架组成。这些BAC库可用于识别和关闭两个物种的基因组组装支架之间的区域。

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