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Functional characterization of pheromone receptors in the tobacco budworm Heliothis virescens

机译:烟草条虫Heliothis virescens中信息素受体的功能表征

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摘要

Functional analyses of candidate Heliothis virescens pheromone odorant receptors (HvORs) were conducted using heterologous expression in Xenopus oocytes. HvOR6 was found to be highly tuned to Z9-14:Ald, while HvOR13, HvOR14 and HvOR16 showed specificity for Z11-16:Ald, Z11-16:OAc and Z11-16:OH, respectively. HvOR15, which had been considered a candidate receptor for Z9-14:Ald did not respond to any of the pheromone compounds tested, nor to 50 other general odorants. Thus, while HvOR15 is specifically expressed in H. virescens male antennae, its role in pheromone reception remains unknown. Based on our results and previous research we can now assign pheromone receptors in H. virescens males to each of the critical H. virescens agonistic pheromone compounds and two antagonistic compounds produced by heterospecific females.
机译:使用非洲爪蟾卵母细胞中的异源表达对候选Heliothis virescens信息素气味受体(HvOR)进行功能分析。发现HvOR6高度调节至Z9-14:Ald,而HvOR13,HvOR14和HvOR16分别显示出对Z11-16:Ald,Z11-16:OAc和Z11-16:OH的特异性。 HvOR15被认为是Z9-14:Ald的候选受体,它对任何测试的信息素化合物或50种其他普通气味均无反应。因此,尽管HvOR15在人纤毛线虫的雄性触角中特异性表达,但其在信息素接收中的作用仍然未知。根据我们的结果和先前的研究,我们现在可以将男性粘膜粘虫中的信息素受体分配给每一种关键的粘膜粘膜芽孢杆菌激动性信息素化合物和两种由异种特异性雌性产生的拮抗化合物。

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