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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Viral replication and host gene expression in alveolar macrophages infected with Ebola virus (Zaire strain).
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Viral replication and host gene expression in alveolar macrophages infected with Ebola virus (Zaire strain).

机译：感染埃博拉病毒（扎伊尔毒株）的肺泡巨噬细胞中的病毒复制和宿主基因表达。

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In order to characterize the cellular response to and identify potential diagnostic markers for the early detection of Ebola virus, an in vitro culture system involving nonhuman primate alveolar macrophages was developed. Ebola virus replication in the alveolar macrophages was characterized by plaque assay, immunohistochemical analysis, and in situ hybridization. Fluorogenic 5'-nuclease assays specific for nonhuman primate proinflammatory cytokines and chemokines were designed and used to evaluate mRNA transcription in macrophages infected with Ebola virus. Transient increases in cytokine and chemokine mRNA levels were observed immediately following exposure to Ebola virus. At 2 h postexposure, levels of cytokine and chemokine mRNAs were markedly reduced. Although Ebola virus infection of alveolar macrophages failed to induce a sustained increase in proinflammatory cytokine and chemokine mRNA transcription (potentially reducing the use of these markers as diagnostic tools), the fluorogenic 5'-nuclease assays developed may have prognostic value for individuals infected with Ebola virus. Recently published data have indicated that persons who remain asymptomatic after exposure to Ebola virus are capable of mounting an early proinflammatory cytokine response and that those who become clinically ill are not. If implemented immediately after exposure, these assays could be used to predict which individuals will be more likely to remain asymptomatic as opposed to those who will be more likely to develop clinical signs and eventually succumb to the virus.
机译：为了表征细胞对埃博拉病毒的反应并确定早期检测埃博拉病毒的潜在诊断标志物，开发了一种涉及非人灵长类动物肺泡巨噬细胞的体外培养系统。通过斑块测定、免疫组织化学分析和原位杂交来表征肺泡巨噬细胞中的埃博拉病毒复制。设计了对非人灵长类促炎细胞因子和趋化因子具有特异性的荧光 5'-核酸酶测定，用于评估感染埃博拉病毒的巨噬细胞的 mRNA 转录。暴露于埃博拉病毒后立即观察到细胞因子和趋化因子mRNA水平的短暂增加。暴露后2小时，细胞因子和趋化因子mRNA水平显著降低。尽管肺泡巨噬细胞的埃博拉病毒感染未能诱导促炎细胞因子和趋化因子mRNA转录的持续增加（可能减少这些标志物作为诊断工具的使用），但开发的荧光5'-核酸酶检测可能对感染埃博拉病毒的个体具有预后价值。最近公布的数据表明，在接触埃博拉病毒后仍然无症状的人能够产生早期促炎细胞因子反应，而那些临床疾病的人则不能。如果在暴露后立即实施，这些检测可用于预测哪些人更有可能保持无症状，而不是那些更有可能出现临床症状并最终屈服于病毒的人。

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