...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads.
【24h】

Kinetic analysis of Smad nucleocytoplasmic shuttling reveals a mechanism for transforming growth factor beta-dependent nuclear accumulation of Smads.

机译：Smad 核质穿梭的动力学分析揭示了转化 Smad 生长因子 β 依赖性核积累的机制。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Upon transforming growth factor beta (TGF-beta) stimulation, Smads accumulate in the nucleus, where they regulate gene expression. Using fluorescence perturbation experiments on Smad2 and Smad4 fused to either enhanced green fluorescent protein or photoactivatable green fluorescent protein, we have studied the kinetics of Smad nucleocytoplasmic shuttling in a quantitative manner in vivo. We have obtained rate constants for import and export of Smad2 and show that the cytoplasmic localization of Smad2 in uninduced cells reflects its nuclear export being more rapid than import. We find that TGF-beta-induced nuclear accumulation of Smad2 is caused by a pronounced drop in the export rate of Smad2 from the nucleus, which is associated with a strong decrease in nuclear mobility of Smad2 and Smad4. TGF-beta-induced nuclear accumulation involves neither a release from cytoplasmic retention nor an increase in Smad2 import rate. Hence, TGF-beta-dependent nuclear accumulation of Smad2 is caused exclusively by selective nuclear trapping of phosphorylated, complexed Smad2. The proposed mechanism reconciles signal-dependent nuclear accumulation of Smad2 with its continuous nucleocytoplasmic cycling properties.
机译：在转化生长因子β（TGF-β）刺激后，Smads在细胞核中积累，在那里它们调节基因表达。通过对融合增强绿色荧光蛋白或光活化绿色荧光蛋白的 Smad2 和 Smad4 的荧光扰动实验，我们研究了 Smad 核质在体内定量穿梭的动力学。我们获得了 Smad2 进出口的速率常数，并表明 Smad2 在非诱导细胞中的细胞质定位反映了其核输出比输入更快。我们发现TGF-β诱导的Smad2的核积累是由Smad2从细胞核输出速率的显着下降引起的，这与Smad2和Smad4的核迁移率的强烈下降有关。TGF-β 诱导的核积累既不涉及细胞质保留的释放，也不涉及 Smad2 输入速率的增加。因此，Smad2 的 TGF-β 依赖性核积累完全是由磷酸化复合物 Smad2 的选择性核捕获引起的。所提出的机制调和了Smad2的信号依赖性核积累与其连续的核质循环特性。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号