...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Detection in on-column conductivity capillary-chip electrophoresis
【24h】

Detection in on-column conductivity capillary-chip electrophoresis

机译：柱上电导率毛细管芯片电泳检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

On-column conductivity detection in capillary-chip electrophoresis was achieved by actively coupling the high electric field with two sensing electrodes connected to the main capillary channel through two side detection channels. The principle of this concept was demonstrated by using a glass chip with a separation channel incorporating two double-Ts. One double-T was used for sample introduction, and the other for detection. The two electrophoresis electrodes apply the high voltage and provide the current, and the two sensing electrodes connected to the separation channel through the second double-T and probe a potential difference. This potential difference is directly related to the local resistance or the conductivity of the solution defined by the two side channels on the main separation channel. A detection limit of 15 mu M (600 ppb or 900 fg) was achieved for potassium ion in a 2 mM Tris-HCl buffer (pH 8.7) with a linear range of 2 orders of magnitude without any stacking. The proposed detection method avoids integrating the sensing electrodes directly within the separation channel and prevents any direct contact of the electrodes with the sample. The baseline signal can also be used for online monitoring of the electric field strength and electroosmosis mobility characterization in the separation channel.
机译：毛细管芯片电泳中的柱上电导率检测是通过将高电场与通过两个侧面检测通道连接到主毛细管通道的两个感应电极主动耦合而实现的。通过使用带有分离通道并结合了两个双T的玻璃芯片证明了这一概念的原理。一个double-T用于样品引入，另一个用于检测。两个电泳电极施加高压并提供电流，两个感测电极通过第二double-T连接到分离通道并探测电势差。该电势差与由主分离通道上的两个侧通道定义的溶液的局部电阻或电导率直接相关。在2 mM的线性范围为2个数量级的Tris-HCl缓冲液（pH 8.7）中，钾离子的检出限达到15μM（600 ppb或900 fg），没有任何堆积。所提出的检测方法避免了将感测电极直接集成在分离通道内，并防止了电极与样品的任何直接接触。基线信号还可用于在线监测分离通道中的电场强度和电渗迁移率表征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号