...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation
【24h】

Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation

机译：通过热固定纤维素衍生物用于尺寸分离蛋白的聚甲基丙烯酸甲酯CE微芯片上的通道壁涂层

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We demonstrate channel wall coating using a cellulose derivative on a poly(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15 degrees) and EOF was significantly suppressed (up to 4.0 x 10(-6) cm(2)center dot V-1 center dot S-1). Three proteins (20.5, 68.0, and 114.6 kDa) were successfully separated on the 0.15% HPMC 90SH-coated channel walls with good reproducibility of migration time (RSD < 1.75%) and high efficiency (theoretical plate number per meter: 2.62 x 10(5)).
机译：我们演示了在聚甲基丙烯酸甲酯（PMMA）CE微芯片上使用纤维素衍生物消除EOF干扰蛋白分离的通道壁涂层。通过用含有纤维素衍生物的溶液进行预处理，然后加热蒸发溶液以产生亲水性通道壁，从而对通道壁进行修饰，从而防止了疏水性相互作用导致分析物的吸附。当PMMA基材上涂有纤维素衍生物羟丙基甲基纤维素（HPMC）90SH时，涂膜基材上的水接触角减小（最大15度），EOF受到显着抑制（最大4.0 x 10（-6）cm（2） ）中心点V-1中心点S-1）。在0.15％HPMC 90SH涂层通道壁上成功分离出三种蛋白质（20.5、68.0和114.6 kDa），具有良好的迁移时间（RSD <1.75％）重现性和高效率（理论每米板数：2.62 x 10（ 5））。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号