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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A micropillar-integrated smart microfluidic device for specific capture and sorting of cells
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A micropillar-integrated smart microfluidic device for specific capture and sorting of cells

机译：集成了微柱的智能微流控设备，用于细胞的特定捕获和分类

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摘要

An integrated smart microfluidic device consisting of nickel micropillars, microvalves, and microchannels was developed for specific capture and sorting of cells. A regular hexagonal array of nickel micropillars was integrated on the bottom of a microchannel by standard photolithography, which can generate strong induced magnetic field gradients under an external magnetic field to efficiently trap superparamagnetic beads (SPMBs) in a flowing stream, forming a bed with sufficient magnetic beads as a capture zone. Fluids could be manipulated by programmed controlling the integrated air-pressure-actuated microvalves, based on which in situ bio-functionalization of SPMBs trapped in the capture zone was realized by covalent attachment of specific proteins directly to their surface on the integrated microfluidic device. In this case, only small volumes of protein solutions (62.5 nL in the capture zone; 3 75 nL in total volume needed to fill the device from inlet A to the intersection of outlet channels F and G) can meet the need for protein! The newly designed microfluidic device reduced greatly chemical and biological reagent consumption and simplified drastically tedious manual handling. Based on the specific interaction between wheat germ agglutinin (WGA) and N-acetylglucosamine on the cell membrane, A549 cancer cells were effectively captured and sorted on the microfluidic device. Capture efficiency ranged from 62 to 74%. The integrated microfluidic device provides a reliable technique for cell sorting.
机译：开发了一种由镍微柱，微阀和微通道组成的集成式智能微流控设备，用于特定的细胞捕获和分选。通过标准光刻将规则的六角形镍微柱阵列集成在微通道底部，该微通道可以在外部磁场下产生强感应磁场梯度，从而有效地将超顺磁珠（SPMB）捕获在流动的流中，从而形成具有足够的床磁珠作为捕获区。可以通过程序控制集成的气压驱动微阀来操纵流体，在此基础上，通过将特定蛋白质直接共价附着到集成微流体装置表面上的特定蛋白质，就可以实现捕获区域捕获的SPMB的原位生物功能化。在这种情况下，只有少量的蛋白质溶液（捕获区域中为62.5 nL;从入口A到出口通道F和G的交叉点填充设备所需的总体积为3 75 nL）才能满足蛋白质的需求！新设计的微流控设备大大减少了化学和生物试剂的消耗，简化了繁琐的人工操作。根据小麦胚芽凝集素（WGA）与N-乙酰氨基葡萄糖之间的特异性相互作用，可以在微流体装置上有效捕获A549癌细胞并进行分类。捕获效率为62％到74％。集成的微流控设备为细胞分选提供了可靠的技术。

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