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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Design, simulation, and optimization of a miniaturized device for size-fractioned DNA extraction
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Design, simulation, and optimization of a miniaturized device for size-fractioned DNA extraction

机译：设计，模拟和优化用于尺寸分级DNA提取的小型设备

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摘要

Fraction collection following electrophoresis has many applications in biology analysis. These assays typically need to identify specific fractions in the separated sample for further processing and require extraction of one or a group of fragments. Unfortunately, the conventional methods involve cumbersome procedures and are not amenable to integration, automation, and extraction of microscale samples. In this work, we present a scheme of chip-based electroelution for the extraction of DNA fragments, which allows the isolation and collection of target DNA fragments in a single device. Both theoretical analysis and experimental results have shown that there are some cross-contaminations from adjacent bands and a little loss of DNA recovery during target DNA extraction with the prototype miniaturized device. In order to improve the purity and yield of the extracted DNA fragment, an optimal channel configuration for DNA recovery miniaturized device was proposed. The simulations and the experimental results are in good agreement and confirm that with the optimized device design a high-efficiency DNA extraction is achieved.
机译：电泳后的馏分收集在生物学分析中有许多应用。这些测定通常需要鉴定分离样品中的特定馏分以进行进一步处理，并且需要提取一个或一组片段。不幸的是，常规方法涉及繁琐的程序，并且不适合于微型样品的整合，自动化和提取。在这项工作中，我们提出了一种用于提取DNA片段的基于芯片的电洗脱方案，该方案允许在单个设备中分离和收集目标DNA片段。理论分析和实验结果均表明，在使用原型小型化设备提取目标DNA的过程中，相邻谱带存在一些交叉污染，并且DNA回收的损失很小。为了提高提取的DNA片段的纯度和产率，提出了用于DNA回收小型化装置的最佳通道构型。仿真结果与实验结果吻合良好，证实了采用优化的设备设计可实现高效的DNA提取。

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