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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A simplified method for capillary embedment into microfluidic devices - exemplified by sol-gel-based preconcentration
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A simplified method for capillary embedment into microfluidic devices - exemplified by sol-gel-based preconcentration

机译：毛细管嵌入微流控设备的简化方法-以基于溶胶-凝胶的预浓缩为例

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摘要

We here describe an alternative method of embedding ftinctionalized capillaries into microdevices fabricated in PDMS. The capillaries have square-shaped outer dimensions and fit into elastic PDMS channel networks of similar dimensions. By modifying the capillary off-chip, the technique makes it possible to integrate a new chip function without risking contamination of already existing chemically patterned areas because of new reagent solutions. Leak-free insertion of these capillaries has earlier been reported, where a thin layer of uncured PDMS bonded the capillary to the microchannel and the lid structure. In this new approach, oxygen plasma is used to bond the square capillary to the PDMS, eliminating the risk of dogging the microsystem with uncured prepolymer. The new embedding technique was exemplified and evaluated by inserting a square capillary piece containing monolithic sol-gel for sample preconcentration application. The assembled microdevice was run with mass spectrometric detection, showing that peptides were preconcentrated without leakage from either the sol-gel itself or around the inserted capillary. Repeated preconcentration runs showed migration times better than 3% for all peptides. We believe that the presented microchip assembling technique greatly simplifies the insertion of functionalized capillary pieces, e.g., an initial preconcentrator to a PDMS device containing other downstream modules.
机译：我们在这里描述了将功能化的毛细管嵌入PDMS中制造的微设备中的另一种方法。毛细管具有方形的外部尺寸，并适合类似尺寸的弹性PDMS通道网络。通过修改芯片外的毛细管，该技术可以集成新的芯片功能，而不会因为新的试剂溶液而冒着污染现有化学图案区域的风险。这些毛细管的无泄漏插入早已有报道，其中未固化的PDMS薄层将毛细管粘合到微通道和盖子结构上。在这种新方法中，氧等离子体用于将方毛细管连接到PDMS，从而消除了用未固化的预聚物缠住微系统的风险。通过插入包含整体式溶胶-凝胶的方形毛细管块，对新的包埋技术进行了示例和评估，以用于样品预浓缩应用。组装的微型设备在质谱检测下运行，表明肽被预浓缩，没有从溶胶凝胶本身或插入的毛细管周围泄漏。重复的预浓缩运行显示所有肽的迁移时间均优于3％。我们相信，提出的微芯片组装技术极大地简化了功能化毛细管件例如初始预浓缩器向包含其他下游模块的PDMS设备的插入。

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