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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Electrodeless direct current dielectrophoresis using reconfigurable field-shaping oil barriers.
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Electrodeless direct current dielectrophoresis using reconfigurable field-shaping oil barriers.

机译:使用可重构场形油障的无电极直流介电电泳。

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摘要

We demonstrate dielectrophoretic (DEP) potential wells using pairs of insulating oil menisci to shape the DC electric field. These oil menisci are arranged in a configuration similar to the quadrupolar electrodes, typically used in DEP, and are shown to produce similar field gradients. While the one-pair well produces a focusing effect on particles in flow, the two-pair well results in creating spatial traps against crossflows. Uncharged polystyrene particles were used to map the DEP force fields and the experimental observations were compared against the field profiles obtained by numerically solving Maxwell's equations. We demonstrate trapping of a single particle due to negative DEP against a pressure-driven crossflow. This can be easily extended to trap and hold cells and other objects against flow for a longer time. We also show the results of particle trapping experiments performed to observe the effect of adjusting the oil menisci and the gap between two pairs of menisci in a four-menisci configuration on the nature of the DEP well formed at the center. A design parameter, Theta, capturing the dimensions of the DEP energy well, is defined and simulations exploring the effects of different geometric features on Theta are presented.
机译:我们展示了使用成对的绝缘油弯液面来塑造DC电场的介电泳(DEP)势阱。这些油弯液面的布置类似于通常在DEP中使用的四极电极,并且显示出产生类似的电场梯度。虽然一对井对流中的粒子产生聚焦作用,但两对井会导致产生针对横流的空间陷阱。使用不带电的聚苯乙烯颗粒绘制DEP力场,并将实验观察结果与通过数值求解麦克斯韦方程组获得的场轮廓进行比较。我们展示了由于负DEP对压力驱动的错流而捕获的单个颗粒。可以很容易地扩展它,以捕获并保持细胞和其他物体防止流动更长的时间。我们还显示了进行粒子捕获实验的结果,以观察调整油弯月面和以四弯月面构型调整两对弯月面之间的间隙对在中心形成的DEP井的性质的影响。定义了一个设计参数Theta来捕获DEP能量井的尺寸,并给出了探索不同几何特征对Theta的影响的仿真。

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