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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Further improvement of hydrostatic pressure sample injection for microchip electrophoresis
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Further improvement of hydrostatic pressure sample injection for microchip electrophoresis

机译：用于微芯片电泳的静水压样品进样的进一步改进

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摘要

Hydrostatic pressure sample injection method is able to minimize the number of electrodes needed for a microchip electrophoresis process; however, it neither can be applied for electrophoretic DNA sizing, nor can be implemented on the widely used single-cross microchip. This paper presents an injector design that makes the hydrostatic pressure sample injection method suitable for DNA sizing. By introducing an assistant channel into the normal double-cross injector, a rugged DNA sample plug suitable for sizing can be successfully formed within the cross area during the sample loading. This paper also demonstrates that the hydrostatic pressure sample injection can be performed in the single-cross microchip by controlling the radial position of the detection point in the separation channel. Rhodamine 123 and its derivative as model sample were successfully separated.
机译：静水压样品注入方法能够使微芯片电泳过程所需的电极数量最少。但是，它既不能用于电泳DNA大小测定，也不能在广泛使用的单交叉微芯片上实现。本文介绍了一种注射器设计，该注射器设计使静水压力样品注射方法适用于DNA尺寸测定。通过将辅助通道引入正常的双交叉进样器，可以在样品加载过程中成功地在交叉区域内形成适合定型的坚固的DNA样品塞。本文还证明，通过控制分离通道中检测点的径向位置，可以在单交叉微芯片中进行静水压力样品注射。罗丹明123及其衍生物作为模型样品已成功分离。

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