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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction.
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Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction.

机译：从葡萄糖反应性和葡萄糖反应性表型对条件培养基的蛋白质组学分析揭示了一组与β细胞功能障碍相关的分泌蛋白。

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摘要

Media conditioned by dysfunctioning pancreatic beta cells offer an excellent source of potential protein markers associated with this phenotype. Proteins identified from cell culture model systems are often found to be of importance clinically. Previous work by us and others have shown that low-passage MIN-6 cells (MIN-6(L)) respond to changes in glucose concentrations, producing an approximately 5.5-fold glucose-stimulated insulin secretion (GSIS) in response to 26.7 mmol/L, compared with 3.3 mmol/L, glucose. After continuous culture or high-passage (MIN-(H)), this GSIS was no longer present and thus represents an excellent model system for investigating beta cell dysfunction. Employing 2-D difference gel electrophoresis and mass spectrometry a panel of protein markers were identified in conditioned media (CM) from MIN-6(L) and MIN-6(H) beta cells. These proteins, including secretogranin II, secretogranin III and transthyretin, are associated with secretory granule biogenesis and were found to have substantially increased levels in the CM from the non-responsive high-passage MIN-6 beta cells. A panel of protein markers found to have increased abundance levels in CM from MIN-6(H) compared with MIN-6(L) beta cells may have the potential to be used clinically for assessing beta cell function and to monitor the effects of specific therapeutics.
机译：由功能异常的胰岛β细胞调节的培养基提供了与此表型相关的潜在蛋白质标记物的极佳来源。从细胞培养模型系统中鉴定出的蛋白质通常在临床上很重要。我们和其他人先前的研究表明，低传代的MIN-6细胞（MIN-6（L））对葡萄糖浓度的变化有反应，对26.7 mmol的反应产生约5.5倍的葡萄糖刺激的胰岛素分泌（GSIS） / L，而葡萄糖为3.3 mmol / L。经过连续培养或高传代（MIN-（H）），此GSIS不再存在，因此代表了研究β细胞功能异常的优秀模型系统。利用二维差异凝胶电泳和质谱，在条件培养基（CM）中从MIN-6（L）和MIN-6（H）β细胞中鉴定出一组蛋白质标记。这些蛋白质，包括促泌素蛋白II，促泌素蛋白III和运甲状腺素蛋白，与分泌性颗粒的生物发生有关，并且发现它们在无反应性高传代MIN-6β细胞中的CM水平显着增加。与MIN-6（L）β细胞相比，一组蛋白质标记物在MIN-6（H）中的CM中的丰度水平提高了，可能具有临床上用于评估β细胞功能和监测特异作用的潜力。疗法。

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