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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Capillary zone electrophoresis of Cowpea mosaic virus and peak identification.
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Capillary zone electrophoresis of Cowpea mosaic virus and peak identification.

机译：Cow豆花叶病毒的毛细管区带电泳和峰鉴定。

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Cowpea mosaic virus (CPMV) was analyzed by CZE and the two separated subcomponents recorded at approximately 2.2 and approximately 2.7 min were respectively designated to be the slow electrophoretic form of CPMV (CPMV(s)) and the fast electrophoretic form of CPMV (CPMV(f)), the two electrophoretic forms of CPMV, by the following methods: first, CZE analysis of the biospecific complexes of CPMV and anti-CPMV polyclonal antibody proved the properties of CPMV; second, isolation and CZE analysis of CPMV(f) and CPMV(s). Because CPMV(s) was precipitated and CPMV(f) was left in the supernatant at pH 5.6, the only one peak at approximately 2.7 min from the supernatant was assigned for CPMV(f), while the peak at approximately 2.2 min dominating for the precipite was deemed to be CPMV(s). Third, viral characterization by directly analyzing the capsid proteins using MALDI-TOF-MS. Based on the theoretical molecular weights calculated from the sequence of the capsid proteins, the three peaks at m/z 21 516.75, 23 745.8 and 42 509.22 for natural CPMV were destined for the small (S) protein of CPMV(f), the S protein of CPMV(s) and the large (L) protein of both of them, respectively. As expected, the non-appearance of the peak at m/z 23 745.8 for the isolated CPMV(f) sample indicated the absence of CPMV(s), and the peak at m/z 23 745.8 was predominant in the spectrum for the precipitated CPMV(s) sample. After the confirmation of CPMV(s) and CPMV(f), the CZE separation of them was optimized. The developed analysis method has proven useful in investigating the stability of CPMV and the effect of 2,4-dinitrophenyl-decoration on the modification of the electrophoretical behavior of CPMV.
机译：通过CZE分析豆花叶病毒（CPMV），分别在约2.2和2.7分钟记录的两个分离的亚组分分别指定为CPMV的慢速电泳形式（CPMV）和CPMV的快速电泳形式（CPMV（ f）），通过以下方法，CPMV的两种电泳形式：首先，对CPMV和抗CPMV多克隆抗体的生物特异性复合物进行CZE分析，证明了CPMV的特性。其次，对CPMV（f）和CPMV（s）进行隔离和CZE分析。由于CPMV沉淀并且CPMV（f）留在pH 5.6的上清液中，因此在距上清液约2.7 min处只有一个峰归属于CPMV（f），而在约2.2 min处的峰占主导地位。沉淀物被认为是CPMV。第三，通过使用MALDI-TOF-MS直接分析衣壳蛋白来表征病毒。根据衣壳蛋白序列计算的理论分子量，天然CPMV的m / z 21 516.75、23 745.8和42 509.22处的三个峰指定为CPMV（f）的小（S）蛋白，S分别是CPMV的蛋白和两个蛋白的大（L）蛋白。不出所料，分离出的CPMV（f）样品在m / z 23 745.8处未出现峰，表明不存在CPMV，而在沉淀谱图中，m / z 23 745.8处的峰为主CPMV样本。在确认了CPMV和CPMV（f）之后，对它们的CZE分离进行了优化。事实证明，开发的分析方法可用于研究CPMV的稳定性以及2,4-二硝基苯基修饰对CPMV电泳行为的影响。

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