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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Separation of acidic and basic proteins by CE with CTAB additive and its applications in peptide and protein profiling.
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Separation of acidic and basic proteins by CE with CTAB additive and its applications in peptide and protein profiling.

机译：带有CTAB添加剂的CE分离酸性和碱性蛋白质及其在肽和蛋白质谱分析中的应用。

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摘要

In this paper, we report a very simple but efficient cationic micellar CE method for protein analysis in complex samples without any modification for the capillary wall. It was found that increased concentration (>or=2 mM) of CTAB additive in electrophoretic buffer can effectively suppress the wall adsorption of both acidic and basic proteins. The separation was improved with buffer pH decreasing from 6.0 to 3.0. By using a buffer containing 2 mM CTAB at pH 3.0, nine proteins with a wide range of pI values (4.7-11.0) were separated within 9 min with high efficiencies (>6 x 10(5) plates per meter) and good reproducibility (RSDs of migration time <0.8% for run-to-run assays) and recoveries (91.6-119.0%). This method was successfully applied to the analysis of complex biological samples, including plasma and red blood cells. Most importantly, we demonstrate the proteomic applications of the proposed method, including the analysis of tryptic digests of BSA and crude protein extracts from Escherichia coli cells. With simplicity, high efficiency, and good reproducibility, this method is promising for protein analysis in complex samples and may find its place in the future proteomic research.
机译：在本文中，我们报告了一种非常简单但有效的阳离子胶束CE方法，可用于复杂样品中的蛋白质分析，而无需对毛细管壁进行任何修改。已发现，在电泳缓冲液中增加CTAB添加剂的浓度（> == 2 mM）可以有效抑制酸性和碱性蛋白质的壁吸附。随着缓冲液pH从6.0降低到3.0，分离得到了改善。通过使用pH 3.0时含有2 mM CTAB的缓冲液，可以在9分钟内以高效率（每米> 6 x 10（5）板）分离9种具有宽pI值（4.7-11.0）的蛋白质。每次运行分析的迁移时间的RSD <0.8％）和回收率（91.6-119.0％）。该方法已成功地应用于复杂生物样品的分析，包括血浆和红细胞。最重要的是，我们证明了所提出方法的蛋白质组学应用，包括分析牛血清白蛋白的胰蛋白酶消化物和大肠杆菌细胞的粗蛋白提取物。该方法简便，高效，重现性好，有望用于复杂样品中的蛋白质分析，并有望在未来的蛋白质组学研究中占有一席之地。

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