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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Equivalence-point electromigration acid-base titration via moving neutralization boundary electrophoresis.
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Equivalence-point electromigration acid-base titration via moving neutralization boundary electrophoresis.

机译：通过移动中和边界电泳进行等价点电迁移酸碱滴定。

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摘要

In this paper, we developed a novel method of acid-base titration, viz. the electromigration acid-base titration (EABT), via a moving neutralization boundary (MNR). With HCl and NaOH as the model strong acid and base, respectively, we conducted the experiments on the EABT via the method of moving neutralization boundary for the first time. The experiments revealed that (i) the concentration of agarose gel, the voltage used and the content of background electrolyte (KCl) had evident influence on the boundary movement; (ii) the movement length was a function of the running time under the constant acid and base concentrations; and (iii) there was a good linearity between the length and natural logarithmic concentration of HCl under the optimized conditions, and the linearity could be used to detect the concentration of acid. The experiments further manifested that (i) the RSD values of intra-day and inter-day runs were less than 1.59 and 3.76%, respectively, indicating similar precision and stability in capillary electrophoresis or HPLC; (ii) the indicators with different pK(a) values had no obvious effect on EABT, distinguishing strong influence on the judgment of equivalence-point titration in the classic one; and (iii) the constant equivalence-point titration always existed in the EABT, rather than the classic volumetric analysis. Additionally, the EABT could be put to good use for the determination of actual acid concentrations. The experimental results achieved herein showed a new general guidance for the development of classic volumetric analysis and element (e.g. nitrogen) content analysis in protein chemistry.
机译：在本文中，我们开发了一种新的酸碱滴定方法，即。通过移动中和边界（MNR）进行电迁移酸碱滴定（EABT）。以HCl和NaOH为模型，分别以强酸和强碱为模型，首次采用移动中和边界的方法对EABT进行了实验。实验表明：（i）琼脂糖凝胶的浓度，使用的电压和背景电解质（KCl）的含量对边界运动有明显的影响; （ii）在恒定的酸和碱浓度下，运动长度是运行时间的函数; （iii）在最佳条件下，HCl的长度与自然对数浓度之间具有良好的线性关系，该线性关系可用于检测酸的浓度。实验进一步表明：（i）日内和日间运行的RSD值分别小于1.59和3.76％，表明在毛细管电泳或HPLC中具有相似的精度和稳定性; （ii）具有不同pK（a）值的指标对EABT的影响不明显，从而对经典点的等效点滴定的判断产生了明显的影响; （iii）EABT中始终存在恒定的等当点滴定，而不是经典的体积分析。另外，EABT可以用于确定实际酸浓度。本文获得的实验结果为蛋白质化学中经典体积分析和元素（例如氮）含量分析的发展提供了新的一般指导。

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