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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.
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Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.

机译:在20 nm高的纳米缝隙中进行电动DNA转运:通过壁吸附运动的证据。

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摘要

The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
机译:研究了在短链PVP存在下,λ-DNA(48 kbp)在20 nm高熔融石英纳米缝隙中的电动迁移行为。流动性和视频数据显示了许多现象,这些现象是DNA通过凝胶或聚合物溶液传输的典型现象,从而表明了DNA途径中的刚性迁移障碍。计算表明,壁吸附的PVP的几纳米薄层(“纳米凝胶”)可以为DNA提供这样的刚性障碍基质。这种超薄壁吸附的聚合物层代表了用于电动DNA分离的新型基质。

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