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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Combinatorial peptide ligand libraries: the conquest of the 'hidden proteome' advances at great strides.
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Combinatorial peptide ligand libraries: the conquest of the 'hidden proteome' advances at great strides.

机译：组合肽配体库：“隐藏蛋白质组”的研究取得了长足的进步。

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The combinatorial peptide ligand library (CPLL) is compared here with the immuno-depletion method for evaluating their respective abilities in digging deeper and deeper into the low-abundance proteome. A recent report suggested in fact that immuno-subtraction for biomarkers discovery in sera does not perform so well, since it results in a meagre 25% increase in identified proteins compared with unfractionated plasma, leaving little capacity to sample lower abundance proteins. On the contrary, CPLLs permit from 300 to 600% increments in detection abilities, as amply demonstrated in several reports. Moreover, when dealing with large sample volumes, an amplification factor of up to four orders of magnitude for trace proteins could be demonstrated, with 80% capture efficiencies even in large (up to 1 L) sample volumes. At present, the lower detection ability of CPLLs has been evaluated at 1 ng/mL (traces of casein additives in white wines).
机译：本文将组合肽配体文库（CPLL）与免疫耗竭方法进行了比较，以评估其各自在向低丰度蛋白质组中深入研究的能力。实际上，最近的一份报告表明，在血清中发现生物标记物的免疫减法效果不佳，因为与未分离的血浆相比，鉴定的蛋白质仅增加了25％，而对较低丰度蛋白质的采样能力却很小。相反，CPLL允许检测能力增加300％至600％，如几份报告中充分证明的那样。此外，当处理大样本量时，痕量蛋白质的扩增因子可以达到四个数量级，即使大样本量（最多1 L），捕获效率也可以达到80％。目前，CPLL的较低检测能力已评估为1 ng / mL（白葡萄酒中的酪蛋白添加剂痕迹）。

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