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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >REPRODUCIBILITY OF POLYPEPTIDE SPOT POSITIONS IN TWO-DIMENSIONAL GELS RUN USING CARRIER AMPHOLYTES IN THE ISOELECTRIC FOCUSING DIMENSION
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REPRODUCIBILITY OF POLYPEPTIDE SPOT POSITIONS IN TWO-DIMENSIONAL GELS RUN USING CARRIER AMPHOLYTES IN THE ISOELECTRIC FOCUSING DIMENSION

机译：在等电聚焦维中使用载流子电泳在二维凝胶中运行的多肽点位置的重现性

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摘要

The reproducibility of complex protein patterns in two-dimensional (2-D) gels run with carrier ampholytes in the first dimension has been investigated, Two different laboratories collaborated in the study and 18 or 19 gels were run in each laboratory for comparison. The electrophoresis chemicals, running devices, and samples were standardized in both labs, The resulting 37 gels were scanned with a charge-coupled device (CCD) camera and spots were located, counted, quantified, and matched using a commercially available image analysis system. Subsequently, the reproducibility of spot position was determined. To perform the statistical analysis, the test gels were initially each matched to a master reference gel. Next, three sets of 12 gels (the image analysis software database could analyze only 12 gels at a time) were analyzed and the isoelectric point (pI) and molecular weight (M-r) positional variation of all the spots that matched across the gels in each set was determined. The resulting statistical analysis indicates very high reproducibility of the carrier ampholyte technique. [References: 10]
机译：已经研究了二维蛋白质（2-D）在带有一维载体两性电解质的凝胶中的复杂蛋白质图案的可再现性，两个不同的实验室在研究中进行了合作，每个实验室分别进行了18或19种凝胶的比较。在两个实验室中，对电泳化学品，电泳仪和样品进行了标准化。使用电荷耦合器件（CCD）相机对所得的37种凝胶进行扫描，并使用市售的图像分析系统对斑点进行了定位，计数，定量和匹配。随后，确定斑点位置的再现性。为了进行统计分析，首先将测试凝胶分别与主参考凝胶匹配。接下来，分析了三组12个凝胶（图像分析软件数据库一次只能分析12个凝胶），并且每个凝胶上匹配的所有斑点的等电点（pI）和分子量（Mr）位置变化确定。所得的统计分析表明载体两性电解质技术具有很高的可重复性。 [参考：10]

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