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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >A TWO-DIMENSIONAL GEL DATABASE OF HUMAN COLON CARCINOMA PROTEINS
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A TWO-DIMENSIONAL GEL DATABASE OF HUMAN COLON CARCINOMA PROTEINS

机译：人结肠癌蛋白质的二维Gel数据库

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The master two-dimensional gel database of human colon carcinoma cells currently lists cellular proteins from normal crypts and the colorectal cancer cell lines LIM 1863, LIM 1215 and LIM 1899 (Ward et al., Electrophoresis 1990, 11, 883-891; Ji et al., Electrophoresis 1994, 15, 391-405). Updated two-dimensional electrophoretic (2-DE) maps of cellular proteins from LIM 1215 cells, acquired under both nonreducing and reducing conditions, are presented. Fifteen cellular proteins are identified in the reducing 2-DE gel map, and seven in the nonreducing gel map, along with a tabular listing of their M-r/pI loci and mode of identification. We also include our mass spectrometric based procedures for identifying 2-DE resolved proteins. This procedure relies on a combination of capillary column (0.10-0.32 mm internal diameter) reversed-phase HPLC pep tide mapping of in-gel digested proteins, peptide mass fingerprinting, sequence analysis by either collision-induced dissociation or post-source-decay fragmentation, and protein identification using available database search algorithms. These data, and descriptions of the micro-techniques employed in this laboratory for identifying 2-DE resolved proteins can be accessed via the internet URL: http://www.ludwig.edu.au. [References: 35]
机译：当前人类结肠癌细胞的二维凝胶主数据库列出了正常隐窝和结直肠癌细胞系LIM 1863，LIM 1215和LIM 1899的细胞蛋白（Ward等人，Electrophoresis 1990，11，883-891; Ji等等，Electrophoresis 1994，15，391-405）。给出了在非还原和还原条件下获得的来自LIM 1215细胞的细胞蛋白的更新的二维电泳图（2-DE）。在还原性2-DE凝胶图中鉴定出15种细胞蛋白，在非还原性凝胶图中鉴定出7种细胞蛋白，以及它们的M-r / pI基因座和鉴定方式的表格列表。我们还包括基于质谱的程序，用于识别2-DE解析的蛋白质。该程序依赖于毛细管柱（内径0.10-0.32 mm）反相HPLC肽图的凝胶内消化蛋白，肽质量指纹图谱，通过碰撞诱导解离或源后衰减片段化进行序列分析的组合以及使用可用的数据库搜索算法进行蛋白质鉴定。这些数据以及该实验室用于鉴定2-DE分解蛋白的微技术的描述可以通过Internet URL：http：//www.ludwig.edu.au访问。 [参考：35]

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