...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells
【24h】

Simultaneous determination of electrophoretic and dielectrophoretic mobilities of human red blood cells

机译：同时测定人红细胞的电泳和介电泳迁移率

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video for real-time observation. The experiment can be performed within 0.044-1 s. In combination with digital calculation based on electromagnetic theory, the method was validated to be applicable to the determination of electrophoretic and dielectrophoretic mobilities, mu(EP) and mu(DEP), of human blood erythrocytes, giving mu(EP) = -(0.87 +/- 0.16) x10(-4) cm(2).V-1.s(-1) and mu(DEP)=-(4.5 +/- 1.3) x10(-8) cm(4).V-2.s(-1) by vector decomposition, or mu(EP)=-(0.89 +/- 0.14) x10(-4) cm(2).V-1.s(-1) and mu(DEP) =-(4.6 +/- 1.2) x10(-8) cm(4).V-2.s(-1) by least squares fitting, all agreeing with published data. Hydrodynamic and EOFs were eliminated for better measurement. It was found that the location of cells had a serious impact on the measurement precision, and the upstream of the cross channel along the electric field was chosen for precise measurement. The method is also extendable to the study of other cells and particles.
机译：细胞的电泳和介电泳可以揭示许多不同的细胞特性，但通常是分开进行的。本文提出了一种同步策略，并通过使细胞通过微视频下的交叉通道迁移进行实时观察来建立一种简单的方法。实验可以在0.044-1 s内完成。与基于电磁理论的数字计算相结合，该方法经验证可用于测定人血红细胞的电泳和介电泳迁移率mu（EP）和mu（DEP），从而mu（EP）=-（0.87 +/- 0.16）x10（-4）cm（2）.V-1.s（-1）和mu（DEP）=-（4.5 +/- 1.3）x10（-8）cm（4）.V-通过向量分解得到2.s（-1），或者mu（EP）=-（0.89 +/- 0.14）x10（-4）cm（2）.V-1.s（-1）和mu（DEP）= -（4.6 +/- 1.2）x10（-8）cm（4）.V-2.s（-1）的最小二乘拟合，均与公开数据一致。消除了流体动力和EOF，以实现更好的测量。发现细胞的位置对测量精度有严重的影响，并且选择沿电场的交叉通道的上游进行精确测量。该方法还可以扩展到其他细胞和颗粒的研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号