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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Peak dispersion and contributions to plate height in nonaqueous capillary electrophoresis at high electric field strengths: Ethanol as background electrolyte solvent
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Peak dispersion and contributions to plate height in nonaqueous capillary electrophoresis at high electric field strengths: Ethanol as background electrolyte solvent

机译：高电场强度下非水毛细管电泳中的峰分散度和对板高的影响：乙醇作为背景电解质溶剂

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摘要

Nonaqueous capillary electrophoretic separations were performed under high electric field strengths (up to 2000 Vcm(-1)) in ethanolic background electrolyte solution and the contributions of different band broadening effects to plate height were evaluated. Under optimum, conditions, increasing the field strength will provide faster separations and increased separation efficiency Decrease in the separation efficiency at high field strengths was, however, observed in a previous study and now in the present paper an attempt is made to quantify various band broadening effects by applying a plate height model, which included the contributions of the injection plug length, diffusion, electro-migration dispersion, Joule heating, analyte adsorption to the capillary wall, and detector slit aperture, length. Of special interest were the contributions of Joule heating and analyte adsorption to the capillary wall. Poly(glycidylmethacrylate-co-N-vinylpyrrolidone)-coated fused-silica capillaries were used with internal diameters (ID) ranging from 30 to 75 mum. The separation efficiencies obtained: experimentally were compared with the theoretically calculated efficiencies and fairly good agreement was observed for the 30 mum ID capillary. Relatively large deviation: from the predictions of the model was founds for the other capillary diameters especially at higher field strengths. The possible, reasons for the deviation: were discussed. [References: 37]
机译：非水毛细管电泳分离是在高电场强度（高达2000 Vcm（-1））下在乙醇背景电解质溶液中进行的，并评估了不同谱带扩展作用对板高的贡献。在最佳条件下，提高场强将提供更快的分离速度，并提高分离效率。但是，在先前的研究中观察到在高场强下分离效率的下降，现在，本文尝试对各种谱带展宽进行量化。通过应用板高度模型可以达到上述效果，该模型包括进样塞长度，扩散，电迁移分散，焦耳加热，分析物吸附到毛细管壁上以及检测器缝隙孔径，长度的影响。特别引起关注的是焦耳加热和分析物吸附到毛细管壁上的作用。使用聚（甲基丙烯酸缩水甘油酯-N-乙烯基吡咯烷酮共聚物）涂覆的熔融二氧化硅毛细管，其内径（ID）为30至75μm。获得的分离效率：将实验与理论计算的效率进行了比较，观察到了30毫米ID毛细管的相当不错的一致性。相对较大的偏差：从模型的预测中发现了其他毛细管直径，尤其是在较高的场强下。讨论了可能的偏差原因： [参考：37]

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