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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >An accessible micro-capillary electrophoresis device using surface-tension-driven flow.
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An accessible micro-capillary electrophoresis device using surface-tension-driven flow.

机译：使用表面张力驱动的流动的可及的微毛细管电泳设备。

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摘要

We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) [G. M. Walker et al., Lab. Chip. 2002, 2, 131-134] injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps, and other equipment typically needed for interfacing with microelectrophoresis chips. This method requires a standard micropipette to load samples before separation, and remove the resulting bands after analysis. The device was made using liquid phase photopolymerization to rapidly fabricate the chip without the need of special equipment typically associated with the construction of microelectrophoresis chips (e.g. cleanroom) [A. K. Agarwal et al., J. Micromech. Microeng. 2006, 16, 332-340; S. K. Mohanty et al., Electrophoresis 2006, 27, 3772-3778]. Batch fabrication time for the device presented here was 1.5 h including channel coating time to suppress electroosmotic flow. Devices were constructed out of poly-isobornyl acrylate and glass. A standard microscope with a UV source was used for sample detection. Separations were demonstrated using Promega BenchTop 100 bp ladder in hydroxyl ethyl cellulose (HEC) and oligonucleotides of 91 and 118 bp were used to characterize sample injection and extraction of DNA bands. The end result was an inexpensive micro-capillary electrophoresis device that uses tools (e.g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users.
机译：我们提出了一种快速制造的微毛细管电泳芯片，该芯片利用表面张力驱动的流动进行样品注射和DNA提取。表面张力驱动的流量（即被动泵送）[G。 M.Walker等人，实验室。芯片。 2002，2，131-134]注入固定体积的样品，该样品可以进行数学预测。被动泵消除了对管道，阀门，注射泵和其他通常需要与微电泳芯片接口的设备的需求。此方法需要使用标准微量移液器在分离前加载样品，并在分析后除去所得谱带。该设备是使用液相光聚合技术快速制造芯片而无需特殊设备的，该设备通常与微电泳芯片的构造（例如无尘室）有关[A. K.Agarwal等人，J.Micromech。 Microeng。 2006，16，332-340; S.K.Mohanty等，Electrophoresis 2006，27，3772-3778]。本文介绍的器件的批量制造时间为1.5小时，其中包括抑制电渗流的通道涂覆时间。装置由丙烯酸聚异冰片酯和玻璃制成。使用带有紫外线源的标准显微镜进行样品检测。使用Promega BenchTop 100 bp阶梯在羟乙基纤维素（HEC）中进行了分离，并使用91和118 bp的寡核苷酸表征了样品注入和DNA条带的提取。最终结果是一种廉价的微毛细管电泳设备，该设备使用了大多数实验室中已经存在的用于样品处理和检测的工具（例如微量移液器，电泳电源和显微镜），使潜在的最终用户更易于使用。

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