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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis.
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Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis.

机译：通过介电泳和磁泳技术快速微流分离磁珠。

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摘要

We present the design and fabrication of a new microfluidic device in which the dielectrophoresis and magnetophoresis phenomena were used for the separation of the superparamagnetic microbeads of different sizes. By exploiting the fact that two different particles can exhibit different dielectrophoretic force-frequency spectra, we utilize this device to perform multiplex detection from a single sample solution. We found the transition frequency range for 1, 2.8, and 4.5 microm magnetic beads using our device. Bead-based analysis revealed that a high separation efficiency ( approximately 90%) could be obtained from a single sample solution containing both 4.5 and 2.8 microm beads. The average flow velocity of the beads was maintained at 9.8 mm/s, enabling fast analysis with a smaller amount of reagents. The magnetic field distribution on the beads and the bead flow at the channel cross section for different dielectrophoretic conditions was obtained using CFD-ACE(+) simulation. Issues relating to the fabrication and operation of the device are discussed in detail. Finally, we demonstrated the feasibility of parallel detection/trapping of different beads on the same chip. This separation approach offers the performance of multiplex analysis in lab-on-a-chip devices.
机译：我们介绍了一种新型的微流控设备的设计和制造，在该设备中，介电电泳和磁泳现象被用于分离不同大小的超顺磁微珠。通过利用两个不同的粒子可以表现出不同的介电泳力-频谱的事实，我们利用该设备从单个样品溶液中进行多重检测。我们使用我们的设备发现了1、2.8和4.5微米磁珠的过渡频率范围。基于珠子的分析表明，从包含4.5和2.8微米珠子的单个样品溶液中可以获得很高的分离效率（大约90％）。珠粒的平均流速保持在9.8 mm / s，可使用少量试剂进行快速分析。使用CFD-ACE（+）模拟获得了不同介电电泳条件下，磁珠上的磁场分布和通道截面处的磁珠流动。详细讨论了与设备的制造和操作有关的问题。最后，我们证明了在同一芯片上并行检测/捕获不同珠子的可行性。这种分离方法可提供片上实验室设备中的多重分析性能。

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