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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >AN ON-LINE TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS PROTEIN DATABASE OF ADULT DROSOPHILA MELANOGASTER
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AN ON-LINE TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS PROTEIN DATABASE OF ADULT DROSOPHILA MELANOGASTER

机译：成人果蝇黑素瘤的二维二维聚丙烯酰胺凝胶电泳蛋白数据库

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An annotated two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) protein database of adult Drosophila melanogaster has been constructed, based on the protein patterns of heads, thoraces and abdomens of adult: male and female Drosophila melanogaster. About 1200 major protein spots are catalogued. Common proteins, found in all body parts, as well as bodypart- and sex-specifically expressed proteins are reported. Of the major proteins, 91, or 7.5%, are differentially expressed in the two sexes or in different body parts, at least in part reflecting specific functional requirements. At the present time 43 proteins, or about 3.5% of the detected proteins, have been identified. These data call be accessed interactively-from our World Wide Web (WWW) server through clickable inline gel images and hypertext: links. Identified protein spots are cross-referenced, through hypertext links, to the SWISS-PROT annotated database of protein primary sequences and the Fly-Base database of Drosophila genomic data. Our reference gels Call be used to gain immediate access to protein spot identify and to the pattern of differentially expressed proteins in Drosophila melanogaster. The work presented in this article ties together information from protein 2-D PAGE, molecular biology and genetics and offers a uniform way to access this large volume of data. [References: 53]
机译：基于成年人的头部，胸部和腹部的蛋白质模式：雄性和雌性果蝇，构建了带注释的成年人果蝇的二维聚丙烯酰胺凝胶电泳（2-D PAGE）蛋白质数据库。约有1200个主要蛋白质斑点被分类。据报道存在于所有身体部位的常见蛋白质，以及特定于身体部位和性别的表达蛋白质。在主要蛋白质中，有91个或7.5％在两个性别或不同的身体部位差异表达，至少部分反映了特定的功能需求。目前，已经鉴定出43种蛋白质，或所检测蛋白质的约3.5％。可通过可单击的行内凝胶图像和超文本链接从我们的万维网（WWW）服务器以交互方式访问这些数据呼叫。通过超文本链接将已识别的蛋白质斑点交叉引用到SWISS-PROT注释的蛋白质主要序列数据库和果蝇基因组数据的Fly-Base数据库。我们的参考凝胶Call可用于立即获得蛋白质斑点鉴定和果蝇中差异表达蛋白质的模式。本文介绍的工作将蛋白质2-D PAGE，分子生物学和遗传学中的信息联系在一起，并提供了一种统一的方式来访问大量数据。 [参考：53]

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