...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >CHARACTERISATION OF WOOL INTERMEDIATE FILAMENT PROTEINS SEPARATED BY MICROPREPARATIVE TWO-DIMENSIONAL ELECTROPHORESIS
【24h】

CHARACTERISATION OF WOOL INTERMEDIATE FILAMENT PROTEINS SEPARATED BY MICROPREPARATIVE TWO-DIMENSIONAL ELECTROPHORESIS

机译：微正向二维电泳分离羊毛中纤丝蛋白的表征

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Wool intermediate filament proteins (IFP) are a subclass of the cytokeratins, a group of structural proteins which form intermediate filaments in many cell types, Post-translational modifications, such as phosphorylation, play an important role in the control of intermediate filament assembly. Two-dimensional electrophoresis has previously been used to study the IFP distribution in wools with different physical characteristics. Charge heterogeneity has been observed in Type I and Type II IFP. In a previous study, two-dimensional electrophoresis of alkaline phosphatase-treated wool protein extracts was used to show that Type II IFP are phosphorylated. To facilitate post-separation analysis, micropreparative two-dimensional electrophoresis was used to separate milligram quantities of wool protein. Direct phosphoamino acid analysis has confirmed the presence of phosphorylation on serine residues on Type II IFP, whose identity was confirmed by amino acid compositional analysis, The isoelectric points of Type I IFP are very similar and they do not separate completely on the commercially available pH 4-7 immobilized pH gradients (IPG) used in this study, In situ tryptic digestion followed by automated Edman sequencing of the. high performance liquid chromatography (HPLC)-separated peptides was used to confirm the identity of this group as Type I IFP. To improve the separation of the Type I IFP it will be necessary to use narrow range IPGs such as Immobiline DryPlates which are available from Pharmacia Biotech, in the pH ranges 4.2-4.9, 4.5-5.4, 5,0-6.0 and 5.6-6.6. [References: 9]
机译：羊毛中间丝蛋白（IFP）是细胞角蛋白的一类，细胞角蛋白是一组结构蛋白，可在许多细胞类型中形成中间丝，翻译后修饰（例如磷酸化）在控制中间丝装配中起重要作用。二维电泳以前已用于研究具有不同物理特性的羊毛中IFP的分布。在I型和II型IFP中观察到电荷异质性。在先前的研究中，碱性磷酸酶处理的羊毛蛋白提取物的二维电泳被用于显示II型IFP被磷酸化。为了促进分离后的分析，使用微量制备的二维电泳分离毫克量的羊毛蛋白。直接的磷酸氨基酸分析已证实II型IFP的丝氨酸残基上存在磷酸化，其身份已通过氨基酸组成分析得到证实。I型IFP的等电点非常相似，并且在市售pH 4上不能完全分离。 -7固定的pH梯度（IPG）用于本研究中，原位胰蛋白酶消化，然后进行自动Edman测序。高效液相色谱（HPLC）分离的肽用于确认该组是否为I型IFP。为了改善I型IFP的分离，必须使用pH范围为4.2-4.9、4.5-5.4、5,0-6.0和5.6-6.6的窄范围IPG，例如可从Pharmacia Biotech获得的Immobiline DryPlate。 。 [参考：9]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号