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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >PVA-based tunable buffering membranes for isoelectric trapping separations.
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PVA-based tunable buffering membranes for isoelectric trapping separations.

机译：基于PVA的可调缓冲膜，用于等电阱分离。

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摘要

PVA-based buffering membranes with tunable pH values were prepared on a PVA substrate by reacting PVA, glycerol-1,3-diglycidyl ether, -NH(2) group-containing buffers and -NH(2) group-containing titrants in the presence of sodium hydroxide. The pH of the buffering membranes could be tuned in the 3H<10 range according to the Hendersson-Hasselbach equation while maintaining buffering capacities in excess of 100 mM. Detailed recipes for the preparation of six families of buffering membranes are tabulated and provided as Supporting Information. The buffering membranes were used to trap and desalt ampholyte solutions and separate proteins having a DeltapI as small as 0.1. The membranes were mechanically and hydrolytically stable and could be stored, even in 10H sodium hydroxide solutions, in excess of 6 months.
机译：通过使PVA，甘油1,3,3-二缩水甘油醚，含-NH（2）基团的缓冲液和含-NH（2）基团的滴定剂在存在下反应，在PVA基板上制备具有可调pH值的基于PVA的缓冲膜氢氧化钠。缓冲膜的pH值可根据Hendersson-Hasselbach方程在3 H <10的范围内调节，同时保持超过100 mM的缓冲能力。列出了制备六种缓冲膜系列的详细配方，并作为支持信息提供。缓冲膜用于捕获和脱盐两性电解质溶液，并分离具有DeltapI小至0.1的蛋白质。该膜具有机械和水解稳定性，即使在10 H的氢氧化钠溶液中也可以保存超过6个月。

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