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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Surface modification of polymer microfluidic devices using in-channel atom transfer radical polymerization.
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Surface modification of polymer microfluidic devices using in-channel atom transfer radical polymerization.

机译：使用通道内原子转移自由基聚合对聚合物微流体装置进行表面改性。

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摘要

In-channel atom transfer radical polymerization (ATRP) was used to graft a PEG layer on the surface of microchannels formed in poly(glycidyl methacrylate)-co-(methyl methacrylate) (PGMAMMA) microfluidic devices. The patterned and cover plates were first anchored with ATRP initiator and then thermally bonded together, followed by pumping a solution containing monomer, catalyst, and ligand into the channel to perform ATRP. A PEG-functionalized layer was grafted on the microchannel wall, which resists protein adsorption. X-ray photoelectron spectroscopy (XPS) was used to investigate the initiator-bound surface, and EOF was measured to evaluate the PEG-grafted PGMAMMA microchannel. Fast, efficient, and reproducible separations of amino acids, peptides, and proteins were obtained using the resultant microdevices. Separation efficiencies were higher than 1.0x10(4) plates for a 3.5 cm separation microchannel. Compared with microdevices modified using a previously reported ATRP technique, these in-channel modified microdevices demonstrated better long-term stability.
机译：通道内原子转移自由基聚合（ATRP）用于将PEG层接枝到聚（甲基丙烯酸缩水甘油酯）-共-（甲基丙烯酸甲酯）（PGMAMMA）微流体装置中形成的微通道表面上。首先将图案化的盖板和盖板用ATRP引发剂固定，然后热粘合在一起，然后将包含单体，催化剂和配体的溶液泵入通道中以进行ATRP。将PEG官能化的层移植到微通道壁上，该层可抵抗蛋白质吸附。使用X射线光电子能谱（XPS）来研究与引发剂结合的表面，并测量EOF来评估PEG接枝的PGMAMMA微通道。使用所得的微型设备可以快速，高效且可重现地分离氨基酸，肽和蛋白质。对于3.5厘米的分离微通道，分离效率高于1.0x10（4）板。与使用先前报道的ATRP技术修改的微型设备相比，这些通道内修改的微型设备表现出更好的长期稳定性。

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